High expression of Cks1 in human non-small cell lung carcinomas
High expression of Cks1 in human non-small cell lung carcinomas
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DOI：
10.1016/s0006-291x(03)00469-8
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发表时间：
2003-04-11
影响因子：
3.1
通讯作者：
Kitagawa, M
中科院分区：
文献类型：
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作者：
Inui, N;Kitagawa, K;Kitagawa, M
细胞周期蛋白依赖性激酶（CDK）抑制剂p27（Kip1）的增强降解已知是许多类型人类癌症的强大预后标志物。人CDK亚基1 （Cks1）和s期激酶相关蛋白2 （Skp2）是SCFSkp2复合物的组成部分，作为p27（Kip1）的泛素连接酶。目前还没有关于Cks1参与人类癌症发病机制的报道。本研究使用Western blotting和实时定量RT-PCR技术发现Cks1在非小细胞肺癌（nsclc）中高表达。Skp2 mRNA表达水平在鳞状细胞癌中较高，且在个别临床样本中与p27（Kip1）蛋白表达水平呈负相关。相比之下，Cks1 mRNA的表达与P27（Kip1）没有这种关系，尽管Cks1 mRNA在腺癌中显著升高。这些结果提示，Skp2和Cks1的高表达可能通过不同的机制参与非小细胞肺癌的发病。(C) 2003 Elsevier Science（美国）版权所有。
Enhanced degradation of cyclin-dependent kinase (CDK) inhibitor p27(Kip1) is known to be a powerful prognostic marker in many types of human cancers. Human CDK subunit 1 (Cks1) and S-phase kinase associated protein 2 (Skp2) are components of the SCFSkp2 complex, which acts as a ubiquitin ligase for p27(Kip1). There are no reports about the involvement of Cks1 in the pathogenesis of human cancer. Here we show high expression of Cks1 in non-small cell lung cancers (NSCLCs) using Western blotting and quantitative real-time RT-PCR. The Skp2 mRNA expression level was high in squamous cell carcinomas and was inversely related with the p27(Kip1) protein level in individual clinical samples. In contrast, Cks1 mRNA expression had no such relationship with P27(Kip1), although Cks1 mRNA was significantly elevated in adenocarcinomas. These results suggest that high expression of Skp2 and Cks1 may be involved in the pathogenesis of NSCLCs via different mechanisms. (C) 2003 Elsevier Science (USA). All rights reserved.