Concurrent measurement of theophylline and caffeine in neonates by an interference-free liquid-chromatographic method.
Concurrent measurement of theophylline and caffeine in neonates by an interference-free liquid-chromatographic method.
复制标题
采用无干扰液相色谱法同时测定新生儿茶碱和咖啡因。
作者：
C. Ou;V. Frawley
建立了6 min内同时测定茶碱和咖啡因含量的灵敏液相色谱法。该方法具有良好的分辨率，可将茶碱、内标和咖啡因与对乙酰氨基酚、乙酰唑胺、乙酰水杨酸盐、氨苄西林、8-氯茶碱、头孢唑林、头孢菌素C、头孢thin、头孢匹林、氯霉素、茶碱、甲硝唑、3-甲基黄嘌呤、水杨酸盐、磺胺嘧啶、磺胺嘧啶、磺胺甲恶唑、磺胺恶唑和可可碱等常见干扰药物完全分离。色谱系统采用Waters' Radial-Pak C18反相柱，以0.1 mol/L磷酸钾缓冲液（pH 4.0, (9.5/90.5 by vol)）为流动相的乙腈为流动相。该方法可检测50微升血清中低至0.5 mg/L的茶碱或咖啡因浓度。精密度和准确度非常好。
A sensitive liquid-chromatographic method was developed for the simultaneous measurement of theophylline and caffeine within 6 min. The excellent resolution of the method allows the complete separation of theophylline, internal standard, and caffeine from frequently encountered interfering drugs including acetaminophen, acetazolamide, acetylsalicylate, ampicillin, 8-chlorotheophylline, cefazolin, cephalosporin C, cephalothin, cephapirin, chloramphenicol, dyphylline, metronidazole, 3-methylxanthine, salicylate, sulfadiazine, sulfamerizine, sulfamethazine, sulfamethoxazole, sulfisoxazole, and theobromine. The chromatographic system involves a Waters' Radial-Pak C18 reversed-phase column and acetonitrile in 0.1 mol/L potassium phosphate buffer, pH 4.0, (9.5/90.5 by vol) as the mobile phase. The method can detect theophylline or caffeine concentrations as low as 0.5 mg/L in 50 microL of serum. Precision and accuracy are excellent.