Screening of genes expressed in developing mandibles during soldier differentiation in the termite Hodotermopsis sjostedti
Screening of genes expressed in developing mandibles during soldier differentiation in the termite Hodotermopsis sjostedti
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DOI：
10.1016/j.febslet.2005.01.031
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发表时间：
2005-02
期刊：
影响因子：
3.5
通讯作者：
Shigeyuki Koshikawa;R. Cornette;M. Hojo;K. Maekawa;Tadao Matsumoto;T. Miura
中科院分区：
文献类型：
--
作者：
Shigeyuki Koshikawa;R. Cornette;M. Hojo;K. Maekawa;Tadao Matsumoto;T. Miura
本文研究了湿木白蚁Hodotermopsis sjostedti在兵蚁分化过程中的形态学变化。利用荧光差异显示技术筛选了在发育中的下颌骨中表达的基因，下颌骨在士兵分化过程中经历了最显著的形态变化。进行序列相似性的数据库搜索，然后通过实时聚合酶链反应定量相对表达水平。在已鉴定的候选基因中，有12个基因在士兵分化过程中表达上调。这些基因包括角质层蛋白、核酸结合蛋白、核糖体蛋白和肌动蛋白结合蛋白，推测这些基因参与了白蚁的等级特异性形态发生。
We investigated the morphological changes accompanying soldier differentiation in the damp-wood termite Hodotermopsis sjostedti. Genes expressed in the developing mandibles, which undergo the most remarkable morphological changes during soldier differentiation, were screened using fluorescent differential display. Database searches for sequence similarities were conducted and the relative expression levels were then quantified by real-time polymerase chain reaction. Among the identified candidate genes, 12 genes were upregulated during soldier differentiation. These included genes for cuticle proteins, nucleic acid binding proteins, ribosomal proteins and actin-binding protein, which were inferred to be involved in caste-specific morphogenesis in termites.