Mining microbial genomes for new natural products and biosynthetic pathways
Mining microbial genomes for new natural products and biosynthetic pathways
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DOI：
10.1099/mic.0.2008/018523-0
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发表时间：
2008-06-01
期刊：
影响因子：
2.8
通讯作者：
Challis, Gregory L.
中科院分区：
文献类型：
--
作者：
Challis, Gregory L.
对微生物基因组序列的分析揭示了许多“神秘”或“孤儿”生物合成基因簇的例子，这些基因簇有可能指导新型、结构复杂的天然产物的生产。本文总结了发现微生物中隐蔽生物合成基因簇产物的各种方法，并介绍了本课题组在模式放线菌天蓝色链霉菌M145中发现的两个这样的基因簇产物。这些发现暗示了天然产物生物合成酶的新机制、作用和特异性。我们的努力来阐明这些描述。新的次生代谢产物的鉴定。腔棘鱼提出了一个问题：它们的生物学功能是什么？还概述了回答这一问题的进展情况。
Analyses of microbial genome sequences have revealed numerous examples of 'cryptic' or 'orphan' biosynthetic gene clusters, with the potential to direct the production of novel, structurally complex natural products. This article summarizes the various methods that have been developed for discovering the products of cryptic biosynthetic gene clusters in microbes and gives an account of my group's discovery of the products of two such gene clusters in the model actinomycete Streptomyces coelicolor M145. These discoveries hint at new mechanisms, roles and specificities for natural product biosynthetic enzymes. Our efforts to elucidate these are described. The identification of new secondary metabolites of S. coelicolor raises the question: what is their biological function? Progress towards answering this question is also summarized.