Microbial communities in liquid and fiber fractions of food waste digestates are differentially resistant to inhibition by ammonia
Microbial communities in liquid and fiber fractions of food waste digestates are differentially resistant to inhibition by ammonia
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DOI：
10.1007/s00253-015-6432-5
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发表时间：
2015-02
影响因子：
5
通讯作者：
W. Peng;Fan Lü;L. Shao;P. He
中科院分区：
文献类型：
--
作者：
W. Peng;Fan Lü;L. Shao;P. He
研究了不同浓度氨（1.0 ~ 7.0 g/L）对纤维和液体消化液中温厌氧消化的影响。采用完整脂质分析方法定量评价纤维和液体消化液内微生物群落的演化，采用DNA指纹图谱法定性评价纤维和液体消化液对氨抑制的抗性。结果表明，总氨氮水平的提高延长了纤维消化的滞后期，降低了液体消化的代谢率。纤维消化液中磷脂脂肪酸（PLFA）浓度比液体消化液高19.6 ~ 50.9倍，而磷脂醚脂（PLEL）浓度仅比液体消化液高2.91 ~ 17.6倍。虽然液体馏分的细胞浓度远低于纤维馏分，但液体消化液的抗氨能力和甲烷化效率均优于纤维消化液。与氨浓度相比，消化菌接种类型对细菌分布的影响更大。主成分分析表明，脂质技术在细菌定量方面优于DNA技术，但检测到的古细菌多样性较少。
The effect of different concentrations of ammonia (1.0–7.0 g/L) during mesophilic anaerobic digestion with fiber or liquid digestate as inoculum was examined. Evolution of microbial community within fiber and liquid digestates was quantitatively assessed by the intact lipid analysis methods and qualitatively by DNA fingerprint methods in order to determine their resistance to ammonia inhibition. The results showed that an increased level of total ammonia nitrogen prolonged the lag phase of fiber digestates while reduced the metabolic rate of liquid digestates. Fiber digestates had 19.6–50.9-fold higher concentrations of phospholipid fatty acids (PLFA) compared to liquid digestates, whereas concentrations of phospholipid ether lipids (PLEL) in the fiber digestates were only 2.91–17.6-fold higher compared to liquid digestates. Although the cell concentration in liquid fraction was far lower than that in the fiber one, the ammonia-resistant ability and the methanization efficiency of the liquid digestate was superior to the fiber digestate. The bacterial profiles were affected more by the type of digestate inoculum compared to the concentration of ammonia. Principal component analysis indicated that the lipids technique was superior to the DNA technique for bacterial quantification but detected less archaeal diversity.