Three-dimensional reconstruction of the human capillary network and the intramyocardial micronecrosis
Three-dimensional reconstruction of the human capillary network and the intramyocardial micronecrosis
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DOI：
10.1152/ajpheart.00486.2010
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发表时间：
2011-03-01
影响因子：
4.8
通讯作者：
Shimamoto, Ken
中科院分区：
文献类型：
--
作者：
Kaneko, Noboru;Matsuda, Ryuko;Shimamoto, Ken
[10]张文龙，王文龙，王文龙，Kaneko N，Matsuda R，户田M，岛本K.人体毛细血管网的三维重建与心肌微坏死。美国生理学杂志心脏循环生理学300：H754-H761，2011年。首次发表于2010年12月10日; doi：10.1152/ajpheart.00486.2010.-对人体心脏进行三维重建，以确定心肌内微血管的结构。从解剖的冠状动脉正常的人心脏的左心室组织制备总共200个连续的连续切片，每个切片6 μ m。使用三维软件重建相应的小动脉、小静脉和所有毛细血管。毛细血管网沿心肌细胞左右沿着延伸，长轴和短轴分别约为130和120 μ m。毛细血管从一条小动脉到邻近的一条小静脉的长度约为350 μ m。毛细血管网中有两种囊状结构，即毛细血管前窦和毛细血管窦，并有许多毛细血管从这些窦分叉出来。心肌细胞表面布满网状毛细血管。相反，毛细血管前窦和毛细血管窦被许多心肌细胞包围。动脉和静脉毛细血管交替排列，形成网格状。存在心肌内微循环单位，形成从两侧小动脉到相邻小静脉的毛细血管网络。心肌微坏死的大小与心肌内微循环单位的大小相对应。这些结果表明，毛细血管网是一个有序的和解剖学调节的结构，微循环单位和毛细血管前窦和毛细血管窦可能在维持心肌内微循环收缩和舒张过程中发挥重要作用。
Kaneko N, Matsuda R, Toda M, Shimamoto K. Threedimensional reconstruction of the human capillary network and the intramyocardial micronecrosis. Am J Physiol Heart Circ Physiol 300: H754-H761, 2011. First published December 10, 2010; doi:10.1152/ajpheart.00486.2010.-Three-dimensional reconstruction of the human heart was performed to define the structure of the intramyocardial microvasculature. A total of 200 consecutive serial sections of 6 mu m each were prepared from the left ventricular tissue of an autopsied human heart with normal coronary arteries. The corresponding arteriole, venule, and all capillaries were reconstructed using three-dimensional software. The capillary network extended right and left along the cardiomyocyte with major and minor axes of about 130 and 120 mu m, respectively. The capillary length from an arteriole to an adjacent venule was about 350 mu m. Two types of sack-like structures, the precapillary sinus and the capillary sinus, were present in the capillary network, and many capillaries diverged from these sinuses. The cardiomyocytes were covered with reticular capillaries. In contrast, the precapillary and capillary sinuses were surrounded by many cardiomyocytes. The arterial and venous capillaries were positioned alternately, forming a lattice pattern. Intramyocardial microcirculatory units forming a capillary network from an arteriole to adjacent venules on both sides were present. The sizes of myocardial micronecroses corresponded to that of the intramyocardial microcirculatory unit. These results show that the capillary network is an ordered and anatomically regulated structure and that the microcirculatory unit and the precapillary and capillary sinuses may play an important role in maintaining the intramyocardial microcirculation during contraction and relaxation.