Proteome-Wide Identification of Lysine Succinylation in the Proteins of Tomato (Solanum lycopersicum).
Proteome-Wide Identification of Lysine Succinylation in the Proteins of Tomato (Solanum lycopersicum).
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番茄（Solanum lycopersicum）蛋白质中赖氨酸琥珀酰化的全蛋白质组鉴定
DOI：
10.1371/journal.pone.0147586
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Wu F
中科院分区：
文献类型：
--
作者：
Jin W;Wu F
赖氨酸琥珀酰化对蛋白质的翻译后修饰在活细胞中起着重要的调节作用。赖氨酸琥珀酰化是最近在大肠杆菌、酵母、弓形虫、HeLa细胞和小鼠肝脏中发现的一种新的翻译后修饰。有趣的是，到目前为止，在植物中只检测到几个赖氨酸琥珀酰化位点。在这项研究中，我们用高分辨率质谱鉴定了番茄202个蛋白中赖氨酸琥珀酰化的347个位点。琥珀酰化蛋白参与多种代谢过程的调节，包括叶绿体和线粒体代谢。生物信息学分析表明，琥珀酰化蛋白具有进化保守性，参与多种细胞功能，如代谢和表观遗传调控。此外，琥珀化蛋白表现出不同的亚细胞定位。我们还定义了六种明确保守的琥珀酰化基序。这些结果首次深入分析了赖氨酸琥珀酰化在番茄中的作用，从而阐明了赖氨酸琥珀酰化在植物细胞生理和代谢物生物合成中的作用。
Post-translational modification of proteins through lysine succinylation plays important regulatory roles in living cells. Lysine succinylation was recently identified as a novel post-translational modification in Escherichia coli, yeast, Toxoplasma gondii, HeLa cells, and mouse liver. Interestingly, only a few sites of lysine succinylation have been detected in plants to date. In this study, we identified 347 sites of lysine succinylation in 202 proteins in tomato by using high-resolution mass spectrometry. Succinylated proteins are implicated in the regulation of diverse metabolic processes, including chloroplast and mitochondrial metabolism. Bioinformatic analysis showed that succinylated proteins are evolutionarily conserved and involved in various cellular functions such as metabolism and epigenetic regulation. Moreover, succinylated proteins exhibit diverse subcellular localizations. We also defined six types of definitively conserved succinylation motifs. These results provide the first in-depth analysis of the lysine succinylome and novel insights into the role of succinylation in tomato, thereby elucidating lysine succinylation in the context of cellular physiology and metabolite biosynthesis in plants.