MEASURING S100 PROTEIN AND NEURON SPECIFIC ENOLASE IN MELANOCYTIC TUMORS USING VIDEO IMAGE-ANALYSIS
MEASURING S100 PROTEIN AND NEURON SPECIFIC ENOLASE IN MELANOCYTIC TUMORS USING VIDEO IMAGE-ANALYSIS
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DOI：
10.1136/jcp.39.10.1096
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发表时间：
1986-10-01
影响因子：
3.4
通讯作者：
JAMAL, O
中科院分区：
文献类型：
--
作者：
WILLIAMS, RA;RODE, J;JAMAL, O
使用计算视频图像分析系统，测量了 19 个恶性黑色素瘤和 16 个良性黑色素细胞病变切片中神经元特异性烯醇化酶 (NSE) 和 S100 蛋白的染色强度。这项研究的结果证实了之前的报道，即 NSE 和 S100 蛋白是恶性黑色素瘤的有用标记物。恶性黑色素瘤的 NSE 染色强度显着高于良性痣 (p = 0.011)。 S100 蛋白的染色强度在恶性黑色素瘤中并没有显着升高。然而，当比较这些肿瘤的浅层和深层皮内部分时，存在显着的 S100 梯度 (p = 0.003)。良性痣中未见此特征。在恶性黑色素瘤的较深部分发现 S100 蛋白染色强度最高。 NSE 染色未观察到这种梯度差异。虽然 NSE 染色的整体强度似乎在区分良性和恶性病变方面更有效，但肿瘤浅层和深层部分之间的染色强度差异可能是恶性肿瘤诊断不确定的病变不良行为的更好指标。
Using a computed video image analysis system, the staining intensity for both neurone specific enolase (NSE) and S100 protein was measured in sections from 19 malignant melanomas and 16 benign melanocytic lesions. The results of this study confirm previous reports that NSE and S100 protein are useful markers for malignant melanoma. NSE staining intensity in the cases of malignant melanoma was significantly higher than that in benign naevi (p = 0.011). Intensity of staining for S100 protein was not significantly higher in the malignant melanomas. There was however, a significant S100 gradient when comparing superficial and deep intradermal portions of these tumours (p = 0.003). This feature was not seen in benign naevi. The greatest intensity of S100 protein staining was found in the deeper portions of the malignant melanomas. This gradient difference was not seen with staining for NSE. Although it seems that the overall intensity of staining for NSE is more effective in differentiating between benign and malignant lesions, the difference in staining intensity between the superficial and deep portions of the tumour may be the better indicator of adverse behaviour in lesions in which the diagnosis of malignancy is uncertain.