ARACHIDONIC-ACID INDUCES LOCAL BUT NOT SYSTEMIC SYNTHESIS OF SALICYLIC-ACID AND CONFERS SYSTEMIC RESISTANCE IN POTATO PLANTS TO PHYTOPHTHORA-INFESTANS AND ALTERNARIA-SOLANI
ARACHIDONIC-ACID INDUCES LOCAL BUT NOT SYSTEMIC SYNTHESIS OF SALICYLIC-ACID AND CONFERS SYSTEMIC RESISTANCE IN POTATO PLANTS TO PHYTOPHTHORA-INFESTANS AND ALTERNARIA-SOLANI
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DOI：
10.1094/phyto-85-1219
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发表时间：
1995-10-01
期刊：
影响因子：
3.2
通讯作者：
METRAUX, JP
中科院分区：
文献类型：
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作者：
COQUOZ, JL;BUCHALA, AJ;METRAUX, JP
当马铃薯幼苗的下部叶片(cv.用多不饱和脂肪酸花生四烯酸(AA)水悬浮液处理后3h，叶片中游离水杨酸(SA)的含量增加了3倍。治疗后6h，血清游离SA水平最高，达3.21g鲜重，对照组为0.15g/g鲜重。处理后3d，处理组叶片中结合态SA含量最高可达51g/g鲜重，而对照组为0.83g/g鲜重。AA处理4d后，上部叶片对马铃薯晚疫病菌产生抗性，但没有观察到SA的积累。这种系统性保护在低至10ppm浓度的AA处理的植物中观察到，不太可能是由于AA在植物中的转移而产生的。此外，AA处理保护马铃薯植株免受早疫病病原菌的侵染，这表明保护范围更广。AA治疗24小时后，观察到病程相关(PR)1类蛋白的局部积聚，而不是全身性积聚。在马铃薯叶片上喷施水杨酸不能提高对致病疫霉的抗性，但在处理24 h后观察到类PR1蛋白的局部积累。健康、感病和抗病马铃薯植株的幼叶中SA的含量高于老叶，这种梯度与其对致病疫霉的天然抗性有关。总的来说，表现田间抗性的品种比感病品种含有更多的结合水杨酸。
When the lower leaves of young potato plants (cv. Bintje) were treated with an aqueous suspension of the polyunsaturated fatty acid arachidonic acid (AA), a threefold increase in the amount of free salicylic acid (SA) in the treated leaves was observed 3 h after treatment. The highest level of free SA was measured 6 h after treatment, reaching 3.21 gig fresh weight compared to 0.15 g/g fresh weight in controls. The levels of conjugated SA in the treated leaves increased up to 51 g/g fresh weight 3 days after treatment compared to 0.83 g/g fresh weight in controls. Four days after treatment with AA the upper leaves became resistant to the potato late blight pathogen, Phytophthora infestans, but no accumulation of SA was observed. This systemic protection was observed in plants treated with AA at concentrations as low as 10 ppm and is unlikely to have arisen from AA translocated in the plant. In addition, treatment with AA protected potato plants against the early blight pathogen, Alternaria solani, indicating a broader spectrum of protection. Local but not systemic accumulation of a pathogenesis-related (PR)1-like protein was observed 24 h after treatment with AA. Application of SA on potato leaves did not confer resistance against P. infestans, but a local accumulation of a PR1-like protein was observed 24 h after treatment. Younger leaves of healthy susceptible and resistant potato plants contained higher levels of SA than older leaves, and this gradient correlated with their natural resistance to P. infestans. In general, cultivars showing field resistance contained higher amounts of conjugated SA than did susceptible cultivars.