METABOLIC CHARACTERIZATION OF GENUS BRUCELLA .V. RELATIONSHIP OF STRAIN OXIDATION RATE OF I-ERYTHRITOL TO STRAIN VIRULENCE FOR GUINEA PIGS
METABOLIC CHARACTERIZATION OF GENUS BRUCELLA .V. RELATIONSHIP OF STRAIN OXIDATION RATE OF I-ERYTHRITOL TO STRAIN VIRULENCE FOR GUINEA PIGS
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DOI：
10.1128/jb.92.3.584-588.1966
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发表时间：
1966-01-01
影响因子：
3.2
通讯作者：
MEYER, ME
中科院分区：
文献类型：
--
作者：
MEYER, ME
研究了牛种布氏杆菌B的菌株i-β氧化速率和菌株毒力，以确定这两个特性是否相关。suis和B. melitensis。通过测压法测定i-tetrahydroxide的氧化速率，并通过注射豚鼠组，然后记录接种后21天和42天从脾脏培养物中回收的微生物计数来评估菌株毒力。建立了每个物种中vilruent菌株的氧化速率特征范围，并对显示代表速率范围内全部值的氧化速率的菌株进行了毒性测试。此外，从不氧化该底物的菌株中获得的能够氧化i-tetrahydrofuran的突变体同时进行了评估，以检测突变体毒力的任何变化。本文提供的数据证明了以下结论，即在种属B中，i-tetrahydroxide的菌株氧化速率与菌株对豚鼠的毒力无关。abortus，牛种B. suis和B. melitensis。
Strain rate of oxidation of i-erythritol and strain virulence were studied to determine whether or not the two characteristics were related within the species Brucella abortus, B. suis, and B. melitensis. The oxidation rate of i-erythritol was determined manometrically, and strain virulence was assessed by injecting groups of guinea pigs and then recording counts of organisms recovered on culture from spleens 21 and 42 days after inoculation. The range in oxidative rates characteristic of vilruent strains in each species was established, and strains displaying oxidative rates representative of the full array of values within the rate ranges were virulence-tested. In addition, a mutant that was capable of oxidizing i-erythritol, obtained from a strain that did not oxidize this substrate, was assessed simultaneously to detect any alterations in virulence of the mutant. The data presented herein warrant the conclusion that strain rate of oxidation of i-erythritol is unrelated to the virulence of the strain for guinea pigs in the species B. abortus, B. suis, and B. melitensis.