In-plane microvortices micromixer-based AC electrothermal for testing drug induced death of tumor cells
In-plane microvortices micromixer-based AC electrothermal for testing drug induced death of tumor cells
复制标题
基于面内微涡流微混合器的交流电热用于测试药物诱导的肿瘤细胞死亡
DOI：
10.1063/1.4967455
复制
发表时间：
2016-11-01
期刊：
影响因子：
3.2
通讯作者：
Jiang, Hongyuan
中科院分区：
文献类型：
--
作者：
Lang, Qi;Ren, Yukun;Jiang, Hongyuan
Herein, we first describe a perfusion chip integrated with an AC electrothermal (ACET) micromixer to supply a uniform drug concentration to tumor cells. The in-plane fluid microvortices for mixing were generated by six pairs of reconstructed novel ACET asymmetric electrodes. To enhance the mixing efficiency, the novel ACET electrodes with rotating angles of 0 degrees, 30 degrees, and 60 degrees were investigated. The asymmetric electrodes with a rotating angle of 60 degrees exhibited the highest mixing efficiency by both simulated and experimental results. The length of the mixing area is 7 mm, and the mixing efficiency is 89.12% (approximate complete mixing) at a voltage of 3V and a frequency of 500 kHz. The applicability of our micromixer with electrodes rotating at 60 degrees was demonstrated by the drug (tamoxifen) test of human breast cancer cells (MCF-7) for five days, which implies that our ACET in-plane microvortices micromixer has great potential for the application of drug induced rapid death of tumor cells and mixing of biomaterials in organs-on-a-chip systems. Published by AIP Publishing.
