Characterization of altered calcium signalling in T lymphocytes from patients with rheumatoid arthritis (RA)
Characterization of altered calcium signalling in T lymphocytes from patients with rheumatoid arthritis (RA)
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DOI：
10.1046/j.1365-2249.1996.d01-768.x
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发表时间：
1996-08-01
影响因子：
4.6
通讯作者：
Young, SP
中科院分区：
文献类型：
--
作者：
Carruthers, DM;Naylor, WG;Young, SP
外周血T淋巴细胞功能异常是RA的特征;观察到体外有丝分裂原刺激后细胞因子的增殖和分泌减少。我们研究了钙流启动T细胞活化类风湿外周血单核细胞（PBMC），以确定是否异常信号也存在。我们发现，与对照组相比，患者PBMC中植物血凝素（PHA-P）和抗CD 3刺激的钙流均显著降低，PHA-P刺激的Ca 2+流率平均差异为6倍（P < 0.01）。当用PHA和CD_3刺激检测纯化的T细胞时，在RA T细胞中观察到峰值和平台[Ca ~（2+）]（i）的降低，但[Ca ~（2+）]（i）的升高速率仅在PHA刺激的那些细胞中降低。这些结果表明，启动信号的改变可能是与RA相关的功能性T细胞异常的基础，并且可能存在来自PBMC群体中非T细胞的额外外在影响。
Abnormal function of peripheral blood T lymphocytes is characteristic of RA; diminished proliferation and secretion of cytokines following in vitro mitogen stimulation are observed. We have investigated the calcium flux initiating T cell activation in rheumatoid peripheral blood mononuclear cells (PBMC) to determine whether abnormalities in signalling are also present. We have found that both phytohaemagglutinin (PHA-P)- and anti-CD3-stimulated calcium fluxes were much reduced in the patients' PBMC compared with controls, with a mean six-fold difference (P < 0.01) in rate of Ca2+ flux with PHA-P stimulation. When purified T cells were examined with PHA and CD3 stimulation, a reduction in the peak and plateau [Ca2+](i) was observed in RA T cells, but the rate of rise of [Ca2+](i) was only reduced in those cells stimulated with PHA. These results suggest that alterations in the initiating signal may underlie the functional T cell abnormalities associated with RA, and that there may be an additional extrinsic influence from non-T cells in the PBMC population.