Effects of Copper Oxide Nanoparticles on the Growth of Rice(Oryza sativa L.) Seedlings and the Relevant Physuological Response
Effects of Copper Oxide Nanoparticles on the Growth of Rice(Oryza sativa L.) Seedlings and the Relevant Physuological Response
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纳米氧化铜对水稻幼苗生长的影响及相关生理反应
DOI：
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发表时间：
2020
影响因子：
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通讯作者：
Ying Gao
中科院分区：
文献类型：
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作者：
Zhongzhou Yang;Yifan Xiao;Tongtong Jiao;Yang Zhang;Jing Chen;Ying Gao
水稻（Oryza sativa L.），作为数十亿人的主要主食，评估了氧化铜纳米颗粒（CuO NPs，尺寸< 50 nm）的植物毒性。在水培条件下，62.5、125和250 mg/L CuO NP处理7 d后，水稻幼苗的生长速率显著低于对照和250 mg/L CuO NP处理。此外，还检测了与抗氧化剂相关的生理指标，包括膜损伤和抗氧化酶活性。250 mg/L CuO纳米粒子处理显著提高了水稻幼苗的丙二醛（MDA）含量和电导率，分别提高了83.4%和67.0%。250 mg/L纳米氧化铜处理水稻叶片的过氧化氢酶和超氧化物歧化酶活性均下降，而125 mg/L纳米氧化铜处理水稻根系的超氧化物歧化酶活性则显著提高了1.66倍。水稻叶片叶绿素a、叶绿素B和类胡萝卜素含量均随CuO-NP处理的进行而降低。最后，为了解释叶绿素变化的潜在分子机制，通过qRT-PCR定量了四个相关基因的表达，即镁螯合酶D亚基、叶绿素合成酶、镁-原卟啉IX甲基转移酶和叶绿素a加氧酶。总体而言，CuO纳米颗粒（特别是250 mg/L浓度）可能通过氧化损伤和干扰叶绿素和类胡萝卜素的合成而影响水稻幼苗的生长发育。
Rice (Oryza sativa L.), a major staple food for billions of people, was assessed for its phytotoxicity of copper oxide nanoparticle (CuO NPs, size < 50 nm). Under hydroponic condition, seven days of exposure to 62.5, 125, and 250 mg/L CuO NPs significantly suppressed the growth rate of rice seedlings compared to both the control and the treatment of supernatant from 250 mg/L CuO NP suspensions. In addition, physiological indexes associated with antioxidants, including membrane damage and antioxidant enzyme activity, were also detected. Treatment with 250 mg/L CuO NPs significantly increased malondialdehyde (MDA) content and electrical conductivity of rice shoots by 83.4% and 67.0%, respectively. The activity of both catalase and superoxide dismutase decreased in rice leaves treated with CuO NPs at the concentration of 250 mg/L, while the activity of the superoxide dismutase significantly increased by 1.66 times in rice roots exposed to 125 mg/L CuO NPs. The chlorophyll, including chlorophyll a and chlorophyll b, and carotenoid content in rice leaves decreased with CuO NP exposure. Finally, to explain potential molecular mechanisms of chlorophyll variations, the expression of four related genes, namely, Magnesium chelatase D subunit, Chlorophyll synthase, Magnesium-protoporphyrin IX methyltransferase, and Chlorophyllide a oxygenase, were quantified by qRT-PCR. Overall, CuO NPs, especially at 250 mg/L concentration, could affect the growth and development of rice seedlings, probably through oxidative damage and disturbance of chlorophyll and carotenoid synthesis.