Prognostic significance of p53, bcl-2, vimentin, and S100 protein-positive Langerhans cells in endometrial carcinoma
Prognostic significance of p53, bcl-2, vimentin, and S100 protein-positive Langerhans cells in endometrial carcinoma
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DOI：
10.1016/s0046-8177(98)90060-0
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发表时间：
1998-05-01
期刊：
影响因子：
3.3
通讯作者：
Jones, M
中科院分区：
文献类型：
--
作者：
Coppola, D;Fu, L;Jones, M
在 50 例子宫内膜癌（6 例 I 期、14 例 II 期、20 例 LII 期和 10 例 Iv 期）中评估了 p53、Bcl2、波形蛋白和 S100 蛋白阳性朗格汉斯细胞的免疫组织化学表达，试图使用这些标记物作为生存预测因子。使用抗生物素蛋白-生物素过氧化物酶复合物技术 (ABC)，将 p53、Bcl-2、波形蛋白和 S100 蛋白的单克隆抗体应用于子宫内膜腺癌的石蜡包埋切片。平均随访 3 年，所有 20 名 I 期和 ZI 期癌症患者均存活。在 30 名 III 期和 IV 期癌症患者中，13 名死于肿瘤（3 年生存率为 57%；SE，10%），8 名携带肿瘤存活，9 名没有肿瘤证据而存活（平均随访 46 个月）。 11 种癌症 (22%) 中存在 p53 强阳性，其中包括 9 种高阶段肿瘤和 2 种低阶段肿瘤。在 33 个肿瘤 (66%) 中发现 Bcl-2 阳性。这些肿瘤大多处于低期；然而，未发现Bcl-2表达与预后之间存在相关性。波形蛋白阳性 (P < .001) 和大量 S100 蛋白阳性朗格汉斯细胞的驯化浸润 (P < .05) 与低期肿瘤相关。 Vimentin 在 23 种癌症中表达，包括 70% 的低期肿瘤和 20% 的高期肿瘤。大多数高级别癌的朗格汉斯细胞已被耗尽；大多数低级别癌显示 >50 个 S100 蛋白阳性朗格汉斯细胞/10 个高倍视野。我们的结果表明朗格汉斯细胞浸润和肿瘤细胞波形蛋白阳性是子宫内膜癌的有利预后因素。版权所有 (C) 1998 W.B.桑德斯公司。
Immunohistochemical expression of p53, Bcl2, vimentin, and S100 protein-positive Langerhans cell was evaluated in 50 endometrial carcinomas (6 stage I, 14 stage II, 20 stage LII, and 10 stage Iv), in an attempt to use these markers as predictors of survival. Monoclonal antibodies to p53, Bcl-2, vimentin, and S100 proteins were applied to paraffin-embedded sections of endometrial adenocarcinoma, using the avidin-biotin peroxidase complex technique (ABC). All 20 patients with stage I and ZI carcinomas were alive with a mean follow-up of 3 years. Of 30 patients with stage III and IV carcinomas, 13 died of tumor (3-year survival, 57%; SE, 10%), eight were alive with tumor, and nine were alive with no evidence of tumor (mean follow-up, 46 months). Strong p53 positivity was present in 11 carcinomas (22%), including nine high-stage and two low-stage tumors. Bcl-2 positivity was identified in 33 tumors (66%). These tumors were mostly low stage; however, no correlation was found between Bcl-2 expression and prognosis. Vimentin positivity (P < .001), and tamer infiltration by a large number of S100 protein-positive Langerhans cells (P < .05) were associated with low-stage tumors. Vimentin was expressed in 23 carcinomas, including 70% of low-stage tumors and 20% of high-stage tumors. Most high-grade carcinomas were Langerhans cell depleted; most low-grade carcinomas showed >50 S100 protein-positive Langerhans cells/10 high-power fields. Our results indicate that Langerhans cell infiltration and vimentin positivity of tumor cells are favorable prognostic factors in endometrial carcinomas. Copyright (C) 1998 by W.B. Saunders Company.