A 3D bioprinting system to produce human-scale tissue constructs with structural integrity
A 3D bioprinting system to produce human-scale tissue constructs with structural integrity
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DOI：
10.1038/nbt.3413
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发表时间：
2016-03-01
影响因子：
46.9
通讯作者：
Atala, Anthony
中科院分区：
文献类型：
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作者：
Kang, Hyun-Wook;Lee, Sang Jin;Atala, Anthony
组织工程的挑战是产生具有临床相关尺寸、形状和结构完整性的三维（3D）血管化细胞构建体。我们提出了一个集成的组织器官打印机（ITOP），可以制造稳定的，人类规模的任何形状的组织结构。机械稳定性是通过将载有细胞的水凝胶与可生物降解的聚合物一起印刷成集成图案并锚定在牺牲水凝胶上来实现的。通过将临床成像数据表示为解剖缺陷的计算机模型并将该模型转化为控制打印机喷嘴运动的程序来实现组织构造的正确形状，打印机喷嘴将细胞分配到离散位置。将微通道结合到组织构建体中有助于营养物扩散到打印的细胞，从而克服了工程化组织中细胞存活的100-200 mm 2的扩散限制。我们通过制造下颌骨和颅骨、软骨和骨骼肌来展示ITOP的能力。ITOP的未来发展方向是生产用于人类的组织，以及构建更复杂的组织和实体器官。
A challenge for tissue engineering is producing three-dimensional (3D), vascularized cellular constructs of clinically relevant size, shape and structural integrity. We present an integrated tissue-organ printer (ITOP) that can fabricate stable, human-scale tissue constructs of any shape. Mechanical stability is achieved by printing cell-laden hydrogels together with biodegradable polymers in integrated patterns and anchored on sacrificial hydrogels. The correct shape of the tissue construct is achieved by representing clinical imaging data as a computer model of the anatomical defect and translating the model into a program that controls the motions of the printer nozzles, which dispense cells to discrete locations. The incorporation of microchannels into the tissue constructs facilitates diffusion of nutrients to printed cells, thereby overcoming the diffusion limit of 100-200 mm for cell survival in engineered tissues. We demonstrate capabilities of the ITOP by fabricating mandible and calvarial bone, cartilage and skeletal muscle. Future development of the ITOP is being directed to the production of tissues for human applications and to the building of more complex tissues and solid organs.