Capture and Genetic Analysis of Circulating Tumor Cells Using a Magnetic Separation Device (Magnetic Sifter).
Capture and Genetic Analysis of Circulating Tumor Cells Using a Magnetic Separation Device (Magnetic Sifter).
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使用磁分离装置（磁筛）捕获循环肿瘤细胞并进行遗传分析。
DOI：
10.1007/978-1-4939-7144-2_12
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Wang,ShanX
中科院分区：
文献类型：
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作者：
Ooi,ChinChun;Park,Seung-Min;Wong,DawsonJ;Gambhir,SanjivS;Wang,ShanX
循环肿瘤细胞（CTC）目前因其作为液体活检一部分的潜在应用而被广泛研究。这些细胞从原发肿瘤脱落到循环系统中，并被认为能够以微创方式深入了解实际肿瘤的分子组成。然而，它们在血液中极为罕见，典型浓度为每毫升血液中 1-100 个；因此，需要一种快速且高纯度的方法从全血中分离CTC。在这里，我们描述了微型磁性筛子以 10 mL/h 的体积流速从全血中分离 CTC 的应用，以及使用基于 PDMS 的纳米井系统进行单细胞基因表达谱分析。该方法可以快速分离 CTC，并随后与下游基因分析方法整合，用于临床应用，例如靶向治疗、治疗监测或进一步的生物学研究。
Circulating tumor cells (CTCs) are currently widely studied for their potential application as part of a liquid biopsy. These cells are shed from the primary tumor into the circulation, and are postulated to provide insight into the molecular makeup of the actual tumor in a minimally invasive manner. However, they are extremely rare in blood, with typical concentrations of 1–100 in a milliliter of blood; hence, a need exists for a rapid and high-purity method for isolating CTCs from whole blood. Here, we describe the application of a microfabricated magnetic sifter toward isolation of CTCs from whole blood at volumetric flow rates of 10 mL/h, along with the use of a PDMS-based nanowell system for single-cell gene expression profiling. This method allows rapid isolation of CTCs and subsequent integration with downstream genetic profiling methods for clinical applications such as targeted therapy, therapy monitoring, or further biological studies.