Cytokines in Blood from Septic Patients Interact With Surface-Immobilized Heparin
Cytokines in Blood from Septic Patients Interact With Surface-Immobilized Heparin
复制标题
脓毒症患者血液中的细胞因子与表面固定肝素相互作用
DOI：
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
O. Larm
中科院分区：
文献类型：
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作者：
J. Axelsson;Mônica S Ferreira;L. Adolfsson;K. Mccrea;R. Ward;O. Larm
当使脓毒症患者的血液通过填充有表面肝素化珠粒的柱时，我们能够显著降低促炎细胞因子肿瘤坏死因子（TNF）的浓度-从最初的非常高的水平。血液通过非肝素化珠不影响TNF水平。同时，调节激活，正常T细胞表达和分泌的白细胞激活细胞因子（RANTES）的浓度保持不变后，通过肝素化的柱，但通过填充有nonheparinized控制珠柱后显着上升。我们的结论是，表面肝素化可能是一个有用的技术，选择性地调节肝素结合细胞因子的水平从全血。这可能对治疗高度炎症性疾病（如严重脓毒症）具有潜在意义。我们的数据还表明，表面活化及其相关的炎症反应可以通过使用体外回路的肝素化来避免。
When passing blood from septic patients through a column packed with surface heparinized beads, we were able to significantly reduce concentrations of the proinflammatory cytokine tumor necrosis factor (TNF)-&agr; from initially very high levels. Passage of blood over nonheparinized beads did not affect the TNF levels. Meanwhile, concentrations of the regulated on activation, normal T-cells expressed, and secreted leukocyte activating cytokine (RANTES) remained unchanged following passage through the heparinized column, but rose significantly after passage through a column packed with the nonheparinized control beads. We conclude that surface heparinization may be a useful technique for selectively regulating the levels of heparin-binding cytokines from whole blood. This may have potential implications for the treatment of hyper-inflammatory conditions such as severe sepsis. Our data also suggests that surface activation and its associated inflammatory response may be avoided by using heparinization of the extracorporeal circuit.