The Morphology and Electrophysiology of the Cochlea of the Miniature Pig
The Morphology and Electrophysiology of the Cochlea of the Miniature Pig
复制标题
小型猪耳蜗的形态和电生理学。
DOI：
10.1002/ar.23095
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发表时间：
2015-03-01
影响因子：
2
通讯作者：
Yang, Shi-Ming
中科院分区：
文献类型：
--
作者：
Guo, Weiwei;Yi, Haijin;Yang, Shi-Ming
目的：报道中国实验小型猪的耳蜗学和电生理学。本研究选用20头中国实验小型猪。记录听性脑干反应(ABR)、耳蜗内淋巴电位(EP)和耳蜗内淋巴钾浓度。用电子显微镜观察毛细胞形态。小型猪的耳蜗囊有三圈半，其中包含一个39 mm长的膜状迷路。迷宫中的Corti器官由三排外毛细胞和一排内毛细胞组成。耳蜗顶周毛细胞的立纤毛明显长于基周毛细胞。前庭器官由三个半规管和耳石器官组成。ABR阈值在4~32 kHz，平均35~45dBSPL(n=20)。1日龄和30日龄小型猪ABR阈值和潜伏期无显著差异。耳蜗术后第2周记录到的平均EP值为77.3±14 mV(n=9)，平均血钾浓度为147.1±13 mm(n=5)。这些对小型猪耳蜗学和电生理学的研究有助于建立中国实验小型猪作为未来耳科学和听力学研究的动物模型。(C)2014年威利期刊公司。
To report the cochlear morphology and electrophysiology of Chinese experimental miniature pigs. Twenty Chinese experimental miniature pigs were used in this study. Auditory brainstem responses (ABR), cochlear endolymphatic potentials (EP), and the potassium concentrations of cochlear endolymph were recorded. Hair cell morphology was examined using electron microscopy. The capsule of cochlea of the miniature pig has three and one-half turns which contains a 39-mm long membranous labyrinth. The organ of Corti in the labyrinth encompasses three rows of outer hair cells and one row of inner hair cells. The stereocilia of the hair cells in the apical turn of the cochlea were significantly longer than those in the basal turn. The vestibular apparatus consists of three semicircular canals and the otolith organs. The average threshold of the ABR was 35-45 dB SPL (n = 20) from 4 to 32 kHz. There was no significant difference in the threshold or latency of the ABR between 1-day-old and 30-day-old miniature pigs. The average EP value was 77.3 +/- 14 mV (n = 9) and the average potassium concentration was 147.1 +/- 13 mM (n = 5) recorded from the second turn of the cochlea. These studies on the cochlear morphology and electrophysiology of the miniature pigs help to establish the Chinese experimental miniature pig as an animal model for future studies in otology and audiology. (C) 2014 Wiley Periodicals, Inc.