The Arabidopsis KH-domain protein FLOWERING LOCUS Y delays flowering by upregulating FLOWERING LOCUS C family members
The Arabidopsis KH-domain protein FLOWERING LOCUS Y delays flowering by upregulating FLOWERING LOCUS C family members
复制标题
拟南芥KH结构域蛋白FLOWERING LOCUS Y通过上调FLOWERING LOCUS C家族成员延迟开花
DOI：
10.1007/s00299-020-02598-w
复制
发表时间：
2020-09-19
影响因子：
6.2
通讯作者：
Yao, Nan
中科院分区：
文献类型：
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作者：
Dai, Guang-Yi;Chen, Ding-Kang;Yao, Nan
我们发现FLY是一种以前未被鉴定的rna结合家族蛋白，它通过正向调节FLC分支成员的表达来控制开花时间。开花植物根据内源和环境信号调整开花时间的能力有助于它们的适应性成功。在拟南芥中，MADS结构域蛋白开花位点C （FLC）和FLC分支成员开花位点M/MADS影响开花1 （FLM/MAF1）、MAF2、MAF3、MAF4和MAF5形成核复合物，在非诱导条件下抑制开花。然而，flm /MAFgenes是如何调控的还需要进一步研究。利用遗传策略，我们发现以前未被鉴定的k同源（KH）结构域蛋白开花位点Y （FLY）调节开花时间。在长日照和短日照条件下，果蝇1基因敲除突变体和果蝇人工microRNA敲除系比野生型更早开花。基因敲除-1等位基因是SALK T-DNA插入突变体，包含由T-DNA整合引起的类似110kb的基因组缺失。FLCclade成员在fly-1突变体和fly-人工microRNA敲低线上被下调，而flcantisense转录水平与野生型相似。我们的研究结果确定了flya是一种通过上调lcclade成员来影响开花时间的调节剂。
Key message We identified FLY as a previously uncharacterized RNA-binding-family protein that controls flowering time by positively regulating the expression of FLC clade members.The ability of flowering plants to adjust the timing of the floral transition based on endogenous and environmental signals contributes to their adaptive success. InArabidopsis thaliana, the MADS-domain protein FLOWERING LOCUS C (FLC) and the FLC clade members FLOWERING LOCUS M/MADS AFFECTING FLOWERING1 (FLM/MAF1), MAF2, MAF3, MAF4, and MAF5 form nuclear complexes that repress flowering under noninductive conditions. However, howFLM/MAFgenes are regulated requires further study. Using a genetic strategy, we showed that the previously uncharacterized K-homology (KH) domain protein FLOWERING LOCUS Y (FLY) modulates flowering time. Thefly-1knockout mutant andFLYartificial microRNA knockdown line flowered earlier than the wild type under long- and short-day conditions. The knockoutfly-1allele, a SALK T-DNA insertion mutant, contains an similar to 110-kb genomic deletion induced by T-DNA integration.FLCclade members were downregulated in thefly-1mutants andFLYartificial microRNA knockdown line, whereas the level of theFLCantisense transcriptCOOLAIRwas similar to that of the wild type. Our results identifyFLYas a regulator that affects flowering time through upregulation ofFLCclade members.