An AFLP, SRAP, and SSR Genetic Linkage Map and Identification of QTLs for Fruit Traits in Pear (Pyrus L.)
An AFLP, SRAP, and SSR Genetic Linkage Map and Identification of QTLs for Fruit Traits in Pear (Pyrus L.)
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DOI：
10.1007/s11105-012-0544-1
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发表时间：
2013-06
影响因子：
2.1
通讯作者：
Rui-ping Zhang;Jun Wu;Xiu-gen Li;M. A. Khan;Hui Chen;S. Korban;Shaoling Zhang
中科院分区：
文献类型：
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作者：
Rui-ping Zhang;Jun Wu;Xiu-gen Li;M. A. Khan;Hui Chen;S. Korban;Shaoling Zhang
本研究利用中国梨种间杂交种八月红(BYH)与中国梨(DS)杂交的97株F1实生苗，建立连锁图谱，进行数量性状基因座(QTL)定位。利用扩增长度多态(AFLP)、简单重复序列(SSR)和序列相关扩增多态(SRAP)标记，结合自交不亲和性基因座，构建了两个亲本连锁图谱。该图谱由143个AFLP、SRAP、6个SSR和S标记组成，位于梨基因组全部17个连锁群上，全长1,352.7 cM。DS图谱由122个标记组成，包括83个AFLP、37个SRAP、1个SSR和S，分布在所有17个连锁群上，覆盖1044.3万cM。根据连续两年(2007年和2008年)对果实重量(克)、果径(厘米)、果长(厘米)、可溶性固形物含量、果形指数和成熟期等6个果实性状的表型数据，检测到19个QTL。这些QTL定位在BYH图谱的LG 01、LG 02、LG 05、LG 07、LG 08、LG 10和DS图谱的LG 02、LG 06、LG 15上，对表型变异的贡献率为7.1~22.0%。以果形指数为主效基因，以果形指数为主效基因，以果实成熟期为主效基因，以克重为单位的主效基因为Pfw-7-1和Pfw-8-1，LOD值≥为3.5。QTLsPfi-8-1、Pfm-8-1和Pfw-8-1与Pfl-8-1共定位在BYH图谱的Lg 08上。在BYH图谱的LG07上，检测到了控制果长、果形指数和果重的QTL。比较两年的QTL位置，果长的Pfl-7-1和Pfl-7-2，果形指数的Pfi-2-1和Pf1-2-2，果实成熟期的Pfm-8-1和Pfm-8-2分别稳定地定位在相同的连锁群上。此外，Pfm-8-1和Pfm-8-2也位于BYH图谱Lg 08的同一区域。
In this study, a population of 97 F1seedlings from a cross between the interspecific hybrid (European × Chinese species) pear ‘Bayuehong’ (BYH) and the Chinese pear ‘Dangshansuli’ (DS) was used for establishing linkage maps and for quantitative trait loci (QTL) discovery. Using amplified length polymorphism (AFLP), simple sequence repeat (SSR), and sequence-related amplified polymorphism (SRAP) markers, along with theSlocus for self-incompatibility, two parental linkage maps were constructed. The map of BYH consisted of 214 markers (143 AFLPs, 64 SRAPs, 6 SSRs, and S) mapped on all 17 linkage groups of the pear genome with a total length of 1,352.7 cM. The map of DS was comprised of 122 markers (83 AFLPs, 37 SRAPs, 1 SSR, and S) distributed along all 17 linkage groups and covering 1,044.3 cM. Based on phenotypic data from two successive years (2007 and 2008) for six fruit traits, including fruit weight (in grams), fruit diameter (in centimeters), fruit length (in centimeters), soluble solids content, fruit shape index, and maturity date, 19 QTLs were detected. These QTLs were mapped on LG 01, LG 02, LG 05, LG 07, LG 08, LG 10 of the BYH map and LG 02, LG 06, LG 15 of the DS map and accounting for 7.1 to 22.0 % of the observed phenotypic variance. Four QTLs,Pfi-8-1for fruit shape index,Pfm-8-1for fruit maturity date,Pfw-7-1andPfw-8-1for fruit weight (in grams), with LOD scores ≥3.5, were deemed as major genes. QTLsPfi-8-1,Pfm-8-1, andPfw-8-1were co-localized on LG 08 of the BYH map, along withPfl-8-1for fruit length. It was observed that on LG 07 of the BYH map, QTLs for fruit length, fruit shape index, and fruit weight were clustered. When QTL locations from both years were compared,Pfl-7-1andPfl-7-2for fruit length,Pfi-2-1andPfi-2-2for fruit shape index, andPfm-8-1andPfm-8-2for fruit maturity date were stably mapped onto the same linkage groups, respectively. Moreover,Pfm-8-1andPfm-8-2were also located within the same region of LG 08 of the BYH map.