INHIBITION OF BACTERIAL-DNA CYTOSINE-5-METHYLTRANSFERASE BY S-ADENOSYL-L-HOMOCYSTEINE AND SOME RELATED-COMPOUNDS
INHIBITION OF BACTERIAL-DNA CYTOSINE-5-METHYLTRANSFERASE BY S-ADENOSYL-L-HOMOCYSTEINE AND SOME RELATED-COMPOUNDS
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DOI：
10.1111/j.2042-7158.1984.tb02999.x
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发表时间：
1984-01-01
影响因子：
3.3
通讯作者：
PINNEY, RJ
中科院分区：
文献类型：
--
作者：
CROOKS, PA;TRIBE, MJ;PINNEY, RJ
S-腺苷-L-高半胱氨酸和5种相关化合物被评价为DNA胞嘧啶-5-甲基转移酶的抑制剂。从大肠杆菌菌株J6-2 dcm+的细胞提取物中检测DNA甲基化，该菌株具有DNA胞嘧啶-5-甲基转移酶活性，含有从大肠杆菌菌株J6 - 2 dcm+中分离的底物DNA。coli菌株J6-2 dcm-1，一株DNA胞嘧啶-5-甲基转移酶缺陷型菌株。在上述酶测定中，S-腺苷-L-高半胱氨酸及其7-脱氮类似物S-杀结核菌素基高半胱氨酸是DNA胞嘧啶-5-甲基转移酶的竞争性抑制剂，Ki分别为14.2和17.6 μ M。
S-Adenosyl-L-homocysteine and 5 related compounds were evaluated as inhibitors of a DNA cytosine-5-methyltransferase. DNA methylation was assayed in cell extracts from Escherichia coli strain J6-2 dcm+, proficient in DNA cytosine-5-methyltransferase activity, containing substrate DNA isolated from E. coli strain J6-2 dcm-, a strain deficient in DNA cytosine-5-methyltransferase. S-Adenosyl-L-homocysteine and its 7-deaza analog, S-tubercidinylhomocysteine, were competitive inhibitors of DNA cytosine-5-methyltransferase with Ki of 14.2 and 17.6 .mu.M, respectively, in the above enzyme assay.