The roles of ERK and P38 MAPK signaling cascades on embryonic diapause initiation and termination of the silkworm, Bombyx mori
The roles of ERK and P38 MAPK signaling cascades on embryonic diapause initiation and termination of the silkworm, Bombyx mori
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DOI：
10.1016/j.ibmb.2005.10.005
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发表时间：
2006-01-01
影响因子：
3.8
通讯作者：
Shiomi, K
中科院分区：
文献类型：
--
作者：
Fujiwara, Y;Shindome, C;Shiomi, K
为了阐明家蚕滞育的分子机制，我们重点研究了油丝裂原活化蛋白激酶（MAPK），这是调节细胞增殖、分化和应激反应的主要组分或信号转导级联。本研究克隆了白细胞外信号相关激酶（ERK）、MAPK-ERK激酶（MEK）和p38 MAPK的cDNA，发现它们的氨基酸序列与其他物种的氨基酸序列有很大的相似性。我们用抗磷酸激酶抗体免疫印迹法分析了这些激酶在滞育起始和终止中的作用。非滞育卵的磷酸化MEK水平始终保持较高水平，滞育卵在滞育阶段后下降，并在转移至5 ℃后45 d开始增加。磷酸化ERK和磷酸化MEK的特征相似，表明ERK磷酸化受MEK调节。在滞育卵中，p38 MAPK磷酸化水平在产卵后36 h开始下降，在5 ℃下15-30 d开始上升，表明p38 MAPK在滞育的开始和结束中起作用。磷酸化ERK水平维持滞育中断治疗和下降滞育维持治疗。ERK磷酸化被认为在滞育终止和发育恢复中起作用。(C)2005爱思唯尔有限公司保留所有权利。
To clarify the molecular mechanisms of Bombyx diapause, we focused oil mitogen-activated protein kinases (MAPK), which are the major components or signal transduction cascades that regulate cell proliferation, differentiation, and stress responses. In the present Study, cloning of Bombyx extracellular signal-related kinase (ERK), MAPK-ERK kinase (MEK), and p38 MAPK cDNA, revealed that their amino acid sequences have close similarity with those of other species. We analyzed the roles of the kinases, in diapause initiation and termination by immuno-blotting with anti-phospho-kinase antibodies. Phospho-MEK levels remained consistently high in non-diapausing eggs, then declined after the diapausing stage in diapausing eggs, and began to increase 45 d after transfer to 5 degrees C Upon diapause termination. The phospho-ERK and phospho-MEK profiles were similar, Suggesting that ERK phosphorylation is regulated by MEK. The phospho-p38 MAPK levels declined 36 h after oviposition in diapausing eggs, and increased at 15-30 d at 5 degrees C in yolk cells, suggesting that p38 MAPK has a role in diapause initiation and termination. Phospho-ERK levels were maintained with diapause-interrupting treatment and declined with diapause-sustaining treatment. ERK phosphorylation is considered to have a role in diapause termination and in the resumption of development. (C) 2005 Elsevier Ltd. All rights reserved.