The concentration-dependent effects of ethanol on Caenorhabditis elegans behaviour
The concentration-dependent effects of ethanol on Caenorhabditis elegans behaviour
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DOI：
10.1038/sj.tpj.6500440
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发表时间：
2007-12-01
影响因子：
2.8
通讯作者：
O'Connor, V.
中科院分区：
文献类型：
--
作者：
Mitchell, P. H.;Bull, K.;O'Connor, V.
乙醇对大脑的影响是浓度依赖性的。低浓度（mM）中毒，而大于100 mM麻醉。与人类酒精成瘾最相关的是中毒机制。以前，秀丽隐杆线虫已被用于遗传筛选，以确定中毒效应。在这里，我们通过提供乙醇在C.秀丽隐翅虫表皮重要的是，乙醇对肌肉活动的影响迅速达到稳态，并且该影响的浓度依赖性在完整动物和暴露肌肉中非常相似。因此，表皮不存在乙醇的吸收屏障，而且内部浓度可能接近外部应用的浓度。因此，在C. elegans需要暴露于小于100mM的外部乙醇。此外，角质层对乙醇的渗透性使得能够分析急性、慢性和间歇性乙醇暴露对行为的精确控制的浓度依赖性影响。
The effects of ethanol on the brain are concentration dependent. Low concentrations (mM) intoxicate, while greater than 100mM anaesthetize. Of most relevance to human alcohol addiction are mechanisms of intoxication. Previously, Caenorhabditis elegans has been employed in genetic screens to define effectors of intoxication. Here, we inform interpretation of these studies by providing evidence that ethanol rapidly equilibriates across C. elegans cuticle. Importantly, the effect of ethanol on muscle activity rapidly reaches steady-state, and the concentration-dependence of the effect is very similar in intact animals and exposed muscle. Thus the cuticle does not present an absorption barrier for ethanol, and furthermore the internal concentration is likely to approach that applied externally. Thus, modelling intoxication in C. elegans requires exposure to external ethanol less than 100mM. Furthermore, the permeability of the cuticle to ethanol enables analysis of precisely controlled concentration-dependent effects of acute, chronic, and episodic ethanol exposure on behaviour.