Development of microsatellite markers and their effectiveness in Lolium temulentum
Development of microsatellite markers and their effectiveness in Lolium temulentum
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DOI：
10.1111/j.1365-3180.2004.00382.x
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发表时间：
2004-04
期刊：
影响因子：
1.7
通讯作者：
T. Senda;N. Kubo;M. Hirai;T. Tominaga
中科院分区：
文献类型：
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作者：
T. Senda;N. Kubo;M. Hirai;T. Tominaga
用合成的(GT)15和(GA)15寡核苷酸筛选天花粉黑麦草基因组文库，我们鉴定了含有微卫星的克隆。根据克隆的序列数据，合成了特异的引物对，并用来自亚洲、非洲和欧洲的10份天冬青和2份桃树材料检验了它们的有效性。15对引物扩增出单一片段，其中5对在天冬青和桃树种质中表现出多态性。在多年生黑麦草(L.perenne)中报道的15对引物也用于本研究。其中9个扩增出微卫星等位基因，其中2个在天冬青和桃树上表现出多态等位基因。多态标记的多态信息含量在0.60~0.86之间，平均为0.76。为天母乳杆菌和多年生乳杆菌开发的微卫星标记在分析种内遗传多样性和系统发育关系方面具有重要意义。
Summary Screening a genomic library of Lolium temulentum (darnel) with synthetic (GT)15 and (GA)15 oligonucleotides, we identified microsatellite-containing clones. Based on the sequence data of the clones, specific primer pairs were synthesized and their effectiveness examined using 10 L. temulentum and two L. persicum accessions from Asia, Africa and Europe. Fifteen primer pairs amplified single fragments, and five of them showed polymorphism in polyacrylamide gel electrophoresis in the L. temulentum and L. persicum accessions. Fifteen primer pairs reported in L. perenne (perennial ryegrass) were also applied in this study. Nine of them amplified the microsatellite alleles, and two of the nine showed polymorphic alleles in L. temulentum and L. persicum. For the polymorphic markers, polymorphic information content values ranged from 0.60 to 0.86 with an average of 0.76 among 10 L. temulentum accessions. The microsatellite markers developed for L. temulentum and L. perenne should be informative and powerful in analysing the intraspecific genetic diversity and the phylogenetic relationships of L. temulentum and L. persicum.