Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions
Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions
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DOI：
10.1109/tnb.2004.837899
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发表时间：
2004-12-01
影响因子：
3.9
通讯作者：
Ober, RJ
中科院分区：
文献类型：
--
作者：
Prabhat, P;Ram, S;Ober, RJ
由于在给定的时间点只能对一个焦平面进行成像，因此使用标准显微镜进行三维细胞动力学成像受到严重限制。在这里，我们提出了对经典显微镜设计的修改，可以同时对两个或多个焦平面进行成像。这是通过修改标准显微镜的发射路径来实现的。通过对珠样品和FcRn-绿色荧光蛋白表达小管进行成像来显示设计的功效，所述FcRn-绿色荧光蛋白表达小管留下分选内体并随后在质膜处胞吐。
The imaging of cellular dynamics in three dimensions using a standard microscope is severely limited due to the fact that only one focal plane can be imaged at a given point in time. Here we present a modification of the classical microscope design with which two or more focal planes can be imaged simultaneously. This is achieved by a modification of the emission pathway of a standard microscope. The efficacy of the design is shown by imaging bead samples and an FcRn-green fluorescent protein expressing tubule that leaves a sorting endosome and subsequently exocytoses at the plasma membrane.