Alcohol Exposure Impacts the Composition of HeLa-Derived Extracellular Vesicles
Alcohol Exposure Impacts the Composition of HeLa-Derived Extracellular Vesicles
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DOI：
10.3390/biomedicines7040078
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发表时间：
2019-12-01
期刊：
影响因子：
4.7
通讯作者：
Matthews, Qiana L.
中科院分区：
文献类型：
--
作者：
Jones, Leandra B.;Kumar, Sanjay;Matthews, Qiana L.
细胞外囊泡是一种纳米级的囊泡，由于其在细胞间通讯中的作用而受到广泛的研究。细胞外囊泡已开始被检查其在疾病保护中的作用以及其作为疾病生物标志物和/或疫苗剂的作用。本研究的目的是探讨酒精暴露对宫颈癌细胞系HeLa细胞外囊泡的生物发生和组成的影响。将HeLa细胞在无外泌体的培养基中培养，并进行模拟处理（对照）或用50 mM或100 mM乙醇处理24小时和48小时。我们的结果表明，酒精显着影响HeLa细胞的活力和外来体生物发生/组成。重要的是，我们的研究证明了酒精对HeLa细胞的关键作用，以及HeLa衍生的细胞外囊泡的生物发生和组成。具体来说，这些结果表明，酒精改变细胞外囊泡的包装热休克蛋白和凋亡蛋白。细胞外囊泡作为HeLa细胞的通讯器，以及疾病发生和进展的生物标志物。
Extracellular vesicles are nanosized vesicles that are under intense investigation for their role in intercellular communication. Extracellular vesicles have begun to be examined for their role in disease protection and their role as disease biomarkers and/or vaccine agents. The goal of this study was to investigate the effects of alcohol exposure on the biogenesis and composition of extracellular vesicles derived from the cervical cancer line, HeLa. The HeLa cells were cultured in exosome-free media and were either mock-treated (control) or treated with 50 mM or 100 mM of alcohol for 24 h and 48 h. Our results demonstrated that alcohol significantly impacts HeLa cell viability and exosome biogenesis/composition. Importantly, our studies demonstrate the critical role of alcohol on HeLa cells, as well as HeLa-derived extracellular vesicle biogenesis and composition. Specifically, these results indicate that alcohol alters extracellular vesicles' packaging of heat shock proteins and apoptotic proteins. Extracellular vesicles serve as communicators for HeLa cells, as well as biomarkers for the initiation and progression of disease.