In vitro methylation of bovine papillomavirus alters its ability to transform mouse cells.
In vitro methylation of bovine papillomavirus alters its ability to transform mouse cells.
复制标题
牛乳头瘤病毒的体外甲基化改变了其转化小鼠细胞的能力。
DOI：
10.1128/mcb.6.8.2910-2915.1986
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发表时间：
1986
影响因子：
5.3
通讯作者：
Scangos,GA
中科院分区：
文献类型：
--
作者：
Christy,BA;Scangos,GA
牛乳头瘤病毒 (BPV) 在体外 29HpaII 位点、27HhaI 位点或两者均被甲基化。 HpaII位点的甲基化使病毒的转化减少了两到六倍，而HhaI位点的甲基化使病毒的转化增加了两到四倍。 HpaII 和 HhaI 位点甲基化的 DNA 与未甲基化的 DNA 在转化效率方面没有明显差异。这些结果表明，特定的甲基化位点，而不是甲基化胞嘧啶残基的绝对水平，在确定对转化的影响方面很重要，并且某些位点甲基化的负面影响可以通过其他位点的甲基化来补偿。甲基化 BPV DNA 转化的细胞中的 BPV 分子几乎不含甲基化，表明甲基化模式并未忠实地保留在这些染色体外复制分子中。克隆的BPV质粒或pBR322中HpaII位点（但不是HhaI位点）的甲基化也抑制质粒转化入大肠杆菌HB101细胞。
Bovine papillomavirus (BPV) was methylated in vitro at either the 29HpaII sites, the 27 HhaI sites, or both. Methylation of theHpaII sites reduced transformation by the virus two- to sixfold, while methylation atHhaI sites increased transformation two- to fourfold. DNA methylated at bothHpaII and HhaI sites did not differ detectably from unmethylated DNA in its efficiency of transformation. These results indicate that specific methylation sites, rather than the absolute level of methylated cytosine residues, are important in determining the effects on transformation and that the negative effects of methylation at some sites can be compensated for by methylation at other sites. BPV molecules in cells transformed by methylated BPV DNA contained little or no methylation, indicating that the pattern of methylation was not faithfully retained in these extrachromosomally replicating molecules. Methylation at theHpaII sites (but not theHhaI sites) in the cloned BPV plasmid or in pBR322 also inhibited transformation of the plasmids intoEscherichia coliHB101 cells.