Contemporary Approaches to the Study of Pain - From Molecules to Neural Networks
Contemporary Approaches to the Study of Pain - From Molecules to Neural Networks
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疼痛研究的当代方法 - 从分子到神经网络
DOI：
10.1007/978-1-0716-2039-7_11
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Hughes D
中科院分区：
文献类型：
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作者：
Hughes D
可视化已识别神经元群的突触连接是定义中枢神经系统神经元回路的重要组成部分。在我们的实验室中，我们优化了几种与共焦和透射电子显微镜（TEM）兼容的组织学方法，以研究脊髓背角的感觉传入、中间神经元和投射神经元之间的解剖关系。本章的目的是提供其中一些方案的详细描述，强调使用这些方法时可能遇到的优点和潜在困难，并参考几项研究，这些研究已应用于研究正常和慢性疼痛状态下的脊髓回路。
Visualizing the synaptic connectivity of identified neuronal populations is an essential part of defining neuronal circuits in the central nervous system. In our laboratory, we have optimized several histological approaches that are compatible with confocal and transmission electron microscopy (TEM) to study the anatomical relationships between sensory afferents, interneurons, and projection neurons in the spinal dorsal horn. The aims of this chapter are to provide detailed descriptions of some of these protocols, to highlight both advantages and potential difficulties that may be encountered when using these approaches, and to reference several studies where these have been applied to study spinal circuits in normal and chronic pain states.