Caspase-3-dependent neuronal death in the hippocampus following kainic acid treatment
Caspase-3-dependent neuronal death in the hippocampus following kainic acid treatment
复制标题
DOI：
10.1016/s0169-328x(99)00143-6
复制
发表时间：
1999-06-18
期刊：
影响因子：
--
通讯作者：
Smeyne, RJ
中科院分区：
文献类型：
--
作者：
Faherty, CJ;Xanthoudakis, S;Smeyne, RJ
在这项研究中，我们检测了敏感小鼠 (FVB/N) 和耐药小鼠 (129/SvEMS) 海马变性红藻氨酸 (KA) 模型中活化的 caspase-3 水平。 KA给药后30小时、2天和4天，处死动物并检查大脑的固缩、TUNEL标记和激活的caspase-3免疫反应性。 KA 处理后 30 小时，在敏感的 FVB/N 菌株中首次检测到具有催化活性的 caspase-3。这是固缩或 TUNEL 标记出现前 18 小时。激活的 caspase-3 的表达持续至注射后 4 天。在耐药性 129/SvEMS 菌株中未检测到激活的 caspase-3 免疫反应性，也没有固缩或 TUNEL 染色的证据。这表明 caspase-3 的激活是 KA 诱导的细胞死亡的必要组成部分。 (C) 1999 Elsevier Science B.V. 保留所有权利。
In this study, we examined the levels of activated caspase-3 in the kainic acid (KA) model of hippocampal degeneration in both sensitive (FVB/N) and resistant (129/SvEMS) strains of mice. At 30 h, 2 and 4 days following KA administration, animals were sacrificed and brains examined for pyknosis, TUNEL labeling, and activated caspase-3 immunoreactivity. Catalytically active caspase-3 was first detected 30 h following KA treatment in the sensitive, FVB/N strain. This was 18 h before the appearance of pyknosis or TUNEL labeling. The expression of activated caspase-3 continues up to 4 days post-injection. No activated caspase-3 immunoreactivity was detected in the resistant, 129/SvEMS strain, neither was there evidence of pyknosis or TUNEL staining. This suggests that activation of caspase-3 is a necessary component of KA-induced cell death. (C) 1999 Elsevier Science B.V. All rights reserved.