In vivo and in vitro antitumor effect of ascorbic acid, lysine, proline, arginine, and green tea extract on human fibrosarcoma cells HT-1080
In vivo and in vitro antitumor effect of ascorbic acid, lysine, proline, arginine, and green tea extract on human fibrosarcoma cells HT-1080
复制标题
抗坏血酸、赖氨酸、脯氨酸、精氨酸和绿茶提取物对人纤维肉瘤细胞HT-1080的体内和体外抗肿瘤作用
作者：
M. Roomi;V. Ivanov;T. Kalinovsky;A. Niedzwiecki;M. Rath
纤维肉瘤是一种侵袭性结缔组织癌症，目前的治疗通常预后不良。基质金属蛋白酶 (MMP)、血管内皮生长因子 (VEGF) 和细胞外基质 (ECM) 的成分（如纤连蛋白）在血管生成中发挥着关键作用，是肿瘤侵袭和转移的基础。赖氨酸、脯氨酸、精氨酸、抗坏血酸和绿茶提取物 (NM) 的这一特性以及抗癌特性促使我们通过测量细胞增殖、MMP-2 和 MMP-9 的调节以及侵袭潜力，在体外研究这些营养素对人纤维肉瘤细胞 HT-1080 的影响。在体内，我们研究了人纤维肉瘤HT-1080细胞在无胸腺裸鼠中的生长以及MMPs和VEGF的表达。通过MTT测定评估细胞增殖，通过明胶酶酶谱评估细胞增殖，通过划痕测定评估细胞侵袭和迁移。对照组和营养补充组均切除肿瘤、称重并进行组织学处理。结果显示，NM抑制了裸鼠肿瘤的生长并缩小了肿瘤的大小；在补充组组织中发现 MMP-9 和 VEGF 分泌减少。 NM 抑制通过基质胶的侵袭和迁移，总抑制浓度为 1000 μg/mL。这些结果为赖氨酸、脯氨酸、精氨酸、抗坏血酸和绿茶提取物的营养混合物在治疗纤维肉瘤中的治疗应用提供了希望。
Current treatment of fibrosarcoma, an aggressive cancer of the connective tissue, is generally associated with poor prognosis. Matrix metalloproteinases (MMPs), vascular endothelial growth factor (VEGF), and constituents of the extracellular matrix (ECM), such as fibronectin, play a critical role in angiogenesis and underlie neoplastic invasion and metastasis. This and anticancer properties of lysine, proline, arginine, ascorbic acid, and green tea extract (NM) prompted us to investigate the effect of these nutrients in vitro on human fibrosarcoma cells HT-1080 by measuring cell proliferation, modulation of MMP-2 and MMP-9, and invasive potential. In vivo, we studied the growth of human fibrosarcoma HT-1080 cells in athymic nude mice and the expression of MMPs and VEGF. Cell proliferation was evaluated by MTT assay, MMP expression by gelatinase zymography, and invasion through Matrigel and migration by scratch assay. Tumors were excised, weighed, and processed for histology in both the control and nutrient-supplemented groups. Results showed NM inhibited the growth and reduced the size of tumors in nude mice; decreased MMP-9 and VEGF secretion was found in the suplemented group tissues. NM inhibited invasion through Matrigel and migration with total inhibition at 1000 μg/mL. These results offer promise in the therapeutic use of the nutrient mixture of lysine, proline, arginine, ascorbic acid, and green tea extract tested in the treatment of fibrosarcoma.
影响因子：
45.3
作者：
Weitz, J;Antonescu, CR;Brennan, MF
通讯作者：
Brennan, MF