Physical characterization of nanoparticle size and surface modification using particle scattering diffusometry
Physical characterization of nanoparticle size and surface modification using particle scattering diffusometry
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DOI：
10.1063/1.4962992
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发表时间：
2016-09-01
期刊：
影响因子：
3.2
通讯作者：
Kinzer-Ursem, Tamara L.
中科院分区：
文献类型：
--
作者：
Clayton, Katherine N.;Salameh, Janelle W.;Kinzer-Ursem, Tamara L.
随着胶体科学领域的不断扩大，用于快速准确地表征胶体颗粒的理化特性的工具将变得越来越重要。在这里，我们提出了粒子散射扩散法（PSD），一种方法，利用暗场显微镜和粒子图像测速的原则，以测量进行布朗运动的粒子的扩散率。PSD测量直径小至30 nm的颗粒的扩散系数，并用于表征作为颗粒的小的、无标记的表面改性的函数的颗粒尺寸和分布的变化。我们证明了快速尺寸的颗粒使用三个数量级的样品体积比目前的标准技术，并使用PSD量化颗粒的均匀性。此外，PSD是足够灵敏的检测纳米厚度的生物分子表面修饰。凭借这些功能，PSD可以可靠地帮助各种应用，包括胶体尺寸，颗粒电晕表征，蛋白质足迹和定量生物分子活性。出版社：AIP Publishing
As the field of colloidal science continues to expand, tools for rapid and accurate physiochemical characterization of colloidal particles will become increasingly important. Here, we present Particle Scattering Diffusometry (PSD), a method that utilizes dark field microscopy and the principles of particle image velocimetry to measure the diffusivity of particles undergoing Brownian motion. PSD measures the diffusion coefficient of particles as small as 30 nm in diameter and is used to characterize changes in particle size and distribution as a function of small, label-free, surface modifications of particles. We demonstrate the rapid sizing of particles using three orders-of-magnitude less sample volume than current standard techniques and use PSD to quantify particle uniformity. Furthermore, PSD is sensitive enough to detect biomolecular surface modifications of nanometer thickness. With these capabilities, PSD can reliably aid in a wide variety of applications, including colloid sizing, particle corona characterization, protein footprinting, and quantifying biomolecule activity. Published by AIP Publishing.