A rice DEAD-box RNA helicase protein, OsRH17, suppresses 16S ribosomal RNA maturation in Escherichia coli.
A rice DEAD-box RNA helicase protein, OsRH17, suppresses 16S ribosomal RNA maturation in Escherichia coli.
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DOI：
10.1016/j.gene.2014.11.025
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发表时间：
2015-01
期刊：
影响因子：
3.5
通讯作者：
Jie Xu;Chaolei Liu;Meiru Li;Jiang Hu;Li Zhu;D. Zeng;Yaolong Yang;Youlin Peng;Ban-pu Ruan;Longbiao Guo;Hongqing Li
中科院分区：
文献类型：
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作者：
Jie Xu;Chaolei Liu;Meiru Li;Jiang Hu;Li Zhu;D. Zeng;Yaolong Yang;Youlin Peng;Ban-pu Ruan;Longbiao Guo;Hongqing Li
死盒蛋白质组成了一个庞大的蛋白质家族。这些蛋白质在RNA代谢的所有类型的过程中起作用，并且在真核生物中高度保守。然而，死亡盒蛋白在水稻生理和发育中的确切功能尚不清楚。OsRH 17是水稻DEAD-box RNA解旋酶中的一个蛋白质，含有DEAD结构域和所有DEAD-box RNA解旋酶的保守基序，在花粉和愈伤组织中特异表达，并受植物激素萘乙酸（NAA）和脱落酸（阿坝）的调控。OsRH 17：GFP融合蛋白定位于细胞核。在原核表达系统中，检测到了OsRH 17的少量表达和部分片段（N-427和C-167）。在E. OsRH 17、N-427和C-167对大肠杆菌的抑制作用与NaCl浓度无关。rRNA基因在E.大肠杆菌中16 SrRNA前体的积累。大肠杆菌细胞的相对生长速率与前16 S rRNA积累水平成反比。结果表明，OsRH 17可能参与了核糖体的生物合成，并抑制了大肠杆菌16 SrRNA的成熟。杆菌在过量表达OsRH 17、N-427和C-167以及OsRH 17敲低的转基因酵母和水稻中没有观察到可见的表型，甚至在一些非生物和生物胁迫下也没有观察到可见的表型，这可能是由于水稻在正常条件下的冗余。
DEAD-box proteins comprise a large protein family. These proteins function in all types of processes in RNA metabolism and are highly conserved among eukaryotes. However, the precise functions of DEAD-box proteins in rice physiology and development remain unclear. In this study, we identified a rice DEAD-box protein, OsRH17, that contains a DEAD domain and all of the common conserved motifs of DEAD-box RNA helicases.OsRH17was specifically expressed in pollen and differentiated callus and upregulated by application of the plant hormones naphthyl acetic acid (NAA) and abscisic acid (ABA). The OsRH17:GFP fusion protein was localized to the nucleus. Tiny amounts of OsRH17 and partial fragments (N-427 and C-167) were detected when they were expressed inEscherichia coli, a prokaryote. Growth of the host cells was suppressed inE. coliby OsRH17, N-427 or C-167, and this suppression was independent of the concentration of the NaCl in the medium. Expression analysis of rRNAs inE. colirevealed that the 16S rRNA precursor accumulated in transgenicE. colicells, and the relative growth rate was inversely proportional to the levels of pre-16S rRNA accumulation. Results suggested that OsRH17 may play a role in ribosomal biogenesis and suppress 16S rRNA maturation inE. coli. No visible phenotype was observed in transgenic yeast and rice (overexpressing OsRH17, N-427, and C-167, as well as OsRH17 knockdown), and even in some abiotic and biotic stresses, which could be due to the redundancy in rice under normal conditions.