Comparative Assessment of Cultures from Oral and Urethral Stem Cells for Urethral Regeneration
Comparative Assessment of Cultures from Oral and Urethral Stem Cells for Urethral Regeneration
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DOI：
10.2174/1574888x10666150902094644
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发表时间：
2016-01-01
影响因子：
2.7
通讯作者：
Pellegrini, Graziella
中科院分区：
文献类型：
--
作者：
Corradini, Francesca;Zattoni, Michela;Pellegrini, Graziella
近年来，由于治疗后尿道狭窄复发等病理原因，尿道重建受到广泛关注。为了获得最佳的风险效益比，已经开发了各种手术技术，例如从口腔中取出的自体移植物。组织工程和干细胞在小活检组织中生长，可以进一步改善手术，减少侵袭性和发病率。为了确定尿道或其他上皮细胞是否同样可用于尿道工程，应比较克隆形成能力、增殖潜力和干细胞标记物。在这项研究中，在重建手术期间从患者身上获取了 19 个尿道活检组织和 21 个口腔粘膜活检组织。从同一供体中取出尿道和口腔组织，以进行原代培养和细胞表征。通过寿命、克隆分析和不同克隆类型的标记物在体外研究了两种组织的长期再生特性。结果显示尿道和口腔粘膜培养物具有相同的高增殖潜力，但保留了特定标记。核型和生长因子依赖性证实了培养细胞的正常表型。增殖室的克隆分析强调了干细胞和瞬时扩增细胞的比例非常不同，其特征是细胞大小概况和标记表达不同。总之，这两种组织都可以在体外培养并保存其干细胞。口腔粘膜与尿道几乎没有差异，这表明它们对于尿道组织工程同样有用。
Urethral reconstruction has received much attention in recent years, due to pathologies such as recurrence of urethral strictures after treatments. Various surgical techniques have been developed to obtain the best risk-benefit ratio, such as autologous grafts taken from the oral cavity. Tissue engineering and stem cells, growing in a tissue from a small biopsies, can further improve surgery, reducing invasiveness and morbidity. To determine whether urethra or other epithelia can be equally useful for urethra engineering, a comparison of clonogenic ability, proliferative potential and stem cell markers should be obtained. In this study, 19 biopsies from urethra, and 21 from oral mucosa were obtained from patients, during reconstructive surgery. Urethral and oral tissues were removed from the same donor, to develop primary cultures and cell characterization. The long term regenerative properties of both tissues were investigated in vitro by life span, clonal analysis and markers of different clonal types. Results revealed the same high proliferative potential for urethra and oral mucosa cultures, but maintenance of specific markers. Karyotype and growth factor dependence confirmed the normal phenotype of cultured cells. Clonal analysis of the proliferative compartment highlighted a very different proportion of stem and transient amplifying cells, characterised by dissimilar cell size profile and marker expression. In conclusion, both tissues can be cultured and preserve their stem cells in vitro. Few differences appeared in oral mucosa vs urethra, suggesting that they can be equally useful for tissue engineering of the urethral tract.