Cloning and expression of a novel human DNA binding protein, PO-GA.
Cloning and expression of a novel human DNA binding protein, PO-GA.
复制标题
新型人类 DNA 结合蛋白 PO-GA 的克隆和表达。
DOI：
10.1006/bbrc.1993.1693
复制
发表时间：
1993
影响因子：
3.1
通讯作者：
Riegel,AT
中科院分区：
文献类型：
--
作者：
Lu,Y;Zeft,AS;Riegel,AT
我们利用转录因子 PO-B 的同源 DNA 结合位点，从 HeLa cDNA 表达文库中克隆了一种新型人类 DNA 结合蛋白。与初始克隆的进一步杂交筛选产生了 4508 bp 的连续 cDNA 序列和编码 128 kDa 蛋白质 PO-GA 的完整开放阅读框。 Northern 分析显示 PO-GA mRNA 在大多数人体组织中广泛分布。然而，肺和肾中的 PO-GA mRNA 水平较低，并且在胎盘组织中检测不到。在大肠杆菌中表达的 PO-GA 的 DNA 结合片段选择性地与 PO-B 元件和其他富含 GA 的双链 DNA 序列以及某些单链 DNA 序列结合。 PO-GA 具有与大肠杆菌和酵母 DNA 连接酶以及参与 DNA 修复的蛋白质同源的区域。因此，除了在转录中发挥潜在作用外，PO-GA 还可能参与 DNA 修复或复制。
We have cloned a novel human DNA-binding protein from a HeLa cDNA expression library using the cognate DNA binding site of a transcription factor, PO-B. Further hybridization screening with the initial clone produced contiguous cDNA sequence of 4508 bp and a complete open reading frame encoding a 128 kDa protein, PO-GA. Northern analysis revealed a wide distribution of PO-GA mRNA in most human tissue. However, PO-GA mRNA levels were lower in lung and kidney and undetectable in placental tissue. A DNA-binding fragment of PO-GA expressed in E. Coli bound selectively to the PO-B element and other GA-rich double-stranded DNA sequences and to certain single-stranded DNA sequences. PO-GA has regions of homology to E. coli and yeast DNA ligases, and to proteins involved in DNA repair. Thus, in addition to a potential role in transcription, PO-GA may also be involved in DNA repair or replication.