Fluorescence microscopy methods in the study of protein structure and function.
Fluorescence microscopy methods in the study of protein structure and function.
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研究蛋白质结构和功能的荧光显微镜方法。
DOI：
10.1007/978-1-59745-523-7_22
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Hallworth,Richard
中科院分区：
文献类型：
--
作者：
Jensen-Smith,Heather;Currall,Benjamin;Rossino,Danielle;Tiede,LeAnn;Nichols,Michael;Hallworth,Richard
As more and more proteins specific to hair cells are discovered, it becomes imperative to understand their structure and how that contributes to their function. The fluorescence microscopic methods described here can be employed to provide information on protein-protein interactions, whether homomeric or heteromeric, and on protein conformation. Here, we describe two fluorescence microscopic methodologies applied to the outer hair cell-specific membrane protein prestin: the intensity and fluorescence lifetime (FLIM) variants of FRET (Fluorescence Resonance Energy Transfer), used in the study of protein-protein interactions, and the Scanning Cysteine Accessibility Method (SCAM), used for the determination of protein conformation. The methods are readily adaptable to other proteins.
