Dietary soy protein containing isoflavonoids does not adversely affect the reproductive tract of male cynomolgus macaques (Macaca fascicularis).
Dietary soy protein containing isoflavonoids does not adversely affect the reproductive tract of male cynomolgus macaques (Macaca fascicularis).
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DOI：
10.1093/jn/137.6.1309
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发表时间：
2007-06
期刊：
影响因子：
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通讯作者：
Donna L. Perry;Jennifer M. Spedick;T. McCoy;M. Adams;A. Franke;J. Cline
中科院分区：
文献类型：
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作者：
Donna L. Perry;Jennifer M. Spedick;T. McCoy;M. Adams;A. Franke;J. Cline
利用啮齿类动物和非人灵长类动物模型，对大豆蛋白衍生的异黄酮进行短期饮食研究，证实了其对生殖道的不同影响。膳食大豆和/或异黄酮对非人灵长类动物生殖道的长期影响尚未确定。本研究的目的是评估长期食用大豆异黄酮对成年雄性食蟹猕猴乳腺、前列腺、睾丸和精子数量的组织形态学的影响。91只成年雄性食蟹猕猴（Macaca fascicularis）在3个月的时间里被喂食蛋白质来源不同的饲料：1)不含大豆、酪蛋白-乳蛋白为基础的饲料或2)低大豆异黄酮含量的饲料（约6毫克）。公斤(1)。D(-1))或3)高大豆异黄酮饮食（约12毫克）。公斤(1)。d(1))。采用液相色谱-光电二极管阵列电喷雾质谱法测定血清中异黄酮的含量，并对死后的乳腺、前列腺和睾丸进行组织病理学和组织形态学检测。进行附睾和睾丸精子计数。饲喂后4 h各组血清中异黄酮浓度差异显著（P < 0.001），分别为（平均值+/- SEM） 67 +/- 23（无大豆日粮）、799 +/- 44（低大豆异黄酮日粮）和1458 +/- 80 nmol。L(-1)（高大豆异黄酮饮食）。饮食没有改变血清雌二醇和睾酮浓度或附睾和睾丸精子计数。各治疗组间脏器重量和组织学指标无差异。乳腺组织病理学和组织形态计量学分析未发现异常，也未发现男性乳房畸形的迹象。我们没有发现大豆异黄酮在生理相关剂量下对成年雄性猕猴生殖器官产生不良影响的证据。
Short-term dietary studies of soy-protein-derived isoflavonoids, using rodent and nonhuman primate models, have documented variable effects on the reproductive tract. Long-term effects of dietary soy and/or isoflavonoids on the reproductive tract of nonhuman primates have not been determined. The objective of this study was to assess the effects of long-term consumption of dietary soy isoflavonoids on histomorphology of the mammary glands and prostate gland, testis, and sperm counts in adult male cynomolgus macaques. Ninety-one adult male cynomolgus macaques (Macaca fascicularis) were fed diets for 3 y differing only in protein source: 1) a soy-free, casein-lactalbumin-based diet or 2) a low-soy isoflavonoid diet ( approximately 6 mg . kg(-1) . d(-1)) or 3) a high-soy isoflavonoid diet ( approximately 12 mg . kg(-1) . d(-1)). Serum isoflavonoids were measured by liquid chromatographic-photodiode array electrospray MS. Mammary gland, prostate gland, and testes were obtained at postmortem and evaluated histopathologically and histomorphometrically. Epididymal and testicular sperm counts were performed. Serum isoflavonoid concentrations at 4 h postfeeding differed among all groups (P < 0.001) and were (means +/- SEM) 67 +/- 23 (soy-free diet), 799 +/- 44 (low-soy isoflavonoid diet), and 1458 +/- 80 nmol . L(-1) (high-soy isoflavonoid diet). Diet did not alter serum estradiol and testosterone concentrations or epididymal and testicular sperm counts. Organ weights and histologic indices did not differ among treatment groups. Mammary gland histopathologic and histomorphometric analysis revealed no abnormalities and no indication of gynecomastia. We found no evidence of an adverse effect of soy isoflavonoids at physiologically relevant doses within the reproductive organs of adult male macaques.