Image-guided tissue engineering of anatomically shaped implants via MRI and micro-CT using injection molding
Image-guided tissue engineering of anatomically shaped implants via MRI and micro-CT using injection molding
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DOI：
10.1089/ten.tea.2007.0186
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发表时间：
2008-07-01
影响因子：
4.1
通讯作者：
Bonassar, Lawrence J.
中科院分区：
文献类型：
--
作者：
Ballyns, Jeffery J.;Gleghorn, Jason P.;Bonassar, Lawrence J.
这项研究首次展示了基于来自广泛使用的医学成像方式(如计算机断层扫描(CT)和磁共振成像(MRI))的解剖几何的工程化组织的发展。计算机辅助设计和组织注射成型技术已经证明了产生复杂几何形状的活体植入物的能力。由于其复杂的几何形状，膝关节的半月板被用作这项技术能力的一个例子。MRI和微型计算机断层扫描(MU CT)被用来设计定制打印的模具，这些模具能够产生解剖形状的结构，在8周的培养过程中保持形状。通过细胞外基质含量和机械性能的增加，工程化结构显示出渐进性的组织形成。界面组织注射成型技术的范例可以应用于其他医学成像技术，呈现解剖的3D模型，展示了将当前技术应用于许多组织和器官工程的潜力。
This study demonstrates for the first time the development of engineered tissues based on anatomic geometries derived from widely used medical imaging modalities such as computed tomography (CT) and magnetic resonance imaging (MRI). Computer-aided design and tissue injection molding techniques have demonstrated the ability to generate living implants of complex geometry. Due to its complex geometry, the meniscus of the knee was used as an example of this technique's capabilities. MRI and microcomputed tomography (mu CT) were used to design custom-printed molds that enabled the generation of anatomically shaped constructs that retained shape throughout 8 weeks of culture. Engineered constructs showed progressive tissue formation indicated by increases in extracellular matrix content and mechanical properties. The paradigm of interfacing tissue injection molding technology can be applied to other medical imaging techniques that render 3D models of anatomy, demonstrating the potential to apply the current technique to engineering of many tissues and organs.