Dietary Polyphenol Supplementation Prevents Alterations of Spatial Navigation in Middle-Aged Mice.
Dietary Polyphenol Supplementation Prevents Alterations of Spatial Navigation in Middle-Aged Mice.
复制标题
DOI：
10.3389/fnbeh.2016.00009
复制
发表时间：
2016
影响因子：
3
通讯作者：
Lafenetre P
中科院分区：
文献类型：
--
作者：
Bensalem J;Servant L;Alfos S;Gaudout D;Layé S;Pallet V;Lafenetre P
在衰老过程中，通常会观察到与海马突触可塑性损伤相关的空间学习和记忆缺陷。此外，膳食多酚的有益作用已被认为是预防这种记忆力下降的潜在功能性食品候选者。事实上，多酚可以增强学习和记忆中突触可塑性的信号通路。在这项研究中，首先根据中年小鼠在莫里斯水迷宫任务中的导航模式来强调和表征中年小鼠的空间学习缺陷。为期八周的富含多酚的饮食，包含从葡萄和蓝莓中提取的富含多酚的提取物（PEGB；来自神经酚联盟），以及高含量的类黄酮、芪和酚酸，随后成功逆转了这些由年龄引起的影响。空间策略的使用确实会随着年龄的增长而延迟，而补充多酚可以促进空间策略的发生。这些行为结果与神经生物学变化相关：虽然中年动物海马钙调蛋白激酶 II (CaMKII) mRNA 的表达水平降低，但富含多酚的饮食可以挽救它们。此外，在补充补充剂的成年和中年小鼠中还观察到神经生长神经营养因子（NGF）mRNA水平的表达增加。因此，这些数据表明，补充多酚可能是预防年龄引起的认知能力下降的有效营养方法。
Spatial learning and memory deficits associated with hippocampal synaptic plasticity impairments are commonly observed during aging. Besides, the beneficial role of dietary polyphenols has been suggested as potential functional food candidates to prevent this memory decline. Indeed, polyphenols could potentiate the signaling pathways of synaptic plasticity underlying learning and memory. In this study, spatial learning deficits of middle-aged mice were first highlighted and characterized according to their navigation patterns in the Morris water maze task. An eight-week polyphenol-enriched diet, containing a polyphenol-rich extract from grape and blueberry (PEGB; from the Neurophenols Consortium) with high contents of flavonoids, stilbenes and phenolic acids, was then successful in reversing these age-induced effects. The use of spatial strategies was indeed delayed with aging whereas a polyphenol supplementation could promote the occurrence of spatial strategies. These behavioral results were associated with neurobiological changes: while the expression of hippocampal calmodulin kinase II (CaMKII) mRNA levels was reduced in middle-aged animals, the polyphenol-enriched diet could rescue them. Besides, an increased expression of nerve growth neurotrophic factor (NGF) mRNA levels was also observed in supplemented adult and middle-aged mice. Thus these data suggest that supplementation with polyphenols could be an efficient nutritional way to prevent age-induced cognitive decline.