Adipose tissue interleukin-18 mRNA and plasma interleukin-18: Effect of obesity and exercise
Adipose tissue interleukin-18 mRNA and plasma interleukin-18: Effect of obesity and exercise
复制标题
DOI：
10.1038/oby.2007.528
复制
发表时间：
2007-02-01
期刊：
影响因子：
6.9
通讯作者：
Pilegaard, Henriette
中科院分区：
文献类型：
--
作者：
Leick, Lotte;Lindegaard, Birgitte;Pilegaard, Henriette
目的：肥胖和缺乏运动的生活方式与发生胰岛素抵抗的风险增加有关。对肥胖与脂肪组织(AT)IL-18mRNA表达增加以及AT IL-18mRNA表达与胰岛素抵抗相关的假设进行了检验。此外，我们推测急性运动和运动训练可以调节AT IL-18mRNA的表达。研究方法和方法：非肥胖者体重指数30 kg/m(2)(女性18例；男性11例)和肥胖者体重指数30 kg/m(2)(女性6例；男性7例)参与研究。分别在安静时、急性运动后即刻、恢复后2小时或10小时采集血样和腹部皮下AT活检标本。肥胖组训练8周后，末次训练后48小时重复采样。结果：肥胖组IL-18 mRNA含量和血浆IL-18浓度均高于非肥胖组(P<0.05)。IL-18mRNA含量和血浆IL-18浓度与胰岛素抵抗呈正相关(p<；0.05)。在研究的时间点，急性运动对IL-18mRNA的表达没有影响，但运动训练使男女的AT IL-18mRNA含量降低了20%。讨论：肥胖和胰岛素抵抗与AT IL-18mRNA和血浆IL-18水平的升高有关，运动诱导的AT IL-18mRNA含量的降低可能有助于有规律的体力活动改善胰岛素敏感性。
Objectives: Obesity and a physically inactive lifestyle are associated with increased risk of developing insulin resistance. The hypothesis that obesity is associated with increased adipose tissue (AT) interleukin (IL)-18 mRNA expression and that AT IL-18 mRNA expression is related to insulin resistance was tested. Furthermore, we speculated that acute exercise and exercise training would regulate AT IL-18 mRNA expression.Research Methods and Procedures: Non-obese subjects with BMI < 30 kg/m(2) (women: n = 18; men; n = 11) and obese subjects with BMI > 30 kg/m(2) (women: n = 6; men: n = 7) participated in the study. Blood samples and abdominal subcutaneous AT biopsies were obtained at rest, immediately after an acute exercise bout, and at 2 hours or 10 hours of recovery. After 8 weeks of exercise training of the obese group, sampling was repeated 48 hours after the last training session.Results: AT IL-18 mRNA content and plasma IL-18 concentration were higher (p < 0.05) in the obese group than in the non-obese group. AT IL-18 mRNA content and plasma IL-18 concentration was positively correlated (p < 0.05) with insulin resistance. While acute exercise did not affect IL-18 mRNA expression at the studied time-points, exercise training reduced AT IL-18 mRNA content by 20% in both sexes.Discussion: Because obesity and insulin resistance were associated with elevated AT IL-18 mRNA and plasma IL-18 levels, the training-induced lowering of AT IL-18 mRNA content may contribute to the beneficial effects of regular physical activity with improved insulin sensitivity.