MORPHOLOGICAL IDENTIFICATION OF THE MOTOR NEURONS INNERVATING THE DORSAL LONGITUDINAL FLIGHT-MUSCLE OF DROSOPHILA-MELANOGASTER
MORPHOLOGICAL IDENTIFICATION OF THE MOTOR NEURONS INNERVATING THE DORSAL LONGITUDINAL FLIGHT-MUSCLE OF DROSOPHILA-MELANOGASTER
复制标题
DOI：
10.1002/cne.902730312
复制
发表时间：
1988-07-15
影响因子：
2.5
通讯作者：
KOENIG, JH
中科院分区：
文献类型：
--
作者：
IKEDA, K;KOENIG, JH
果蝇的背侧纵向飞行肌（DLM）由六根肌纤维（DLM1-6从腹侧到背侧）组成，由五个运动神经元（MN）支配，DLM1-4由MNI-4单独支配，DLM5和6由MN5共同支配。这项研究识别并描述了支配这六种肌纤维的五个运动神经元。在细胞内将辣根过氧化物酶 (HRP) 应用于单个已识别的 DLM 纤维，导致通过逆行突触转运来标记支配该肌纤维的单个运动神经元。该方法允许对支配特定肌纤维的运动神经元进行积极识别，因为仅标记了支配神经元。追踪胸神经节中每个标记运动神经元的轴突通路、胞体和树突分布，并确定它们的相对位置。 MN1-4 的体细胞位于胸神经节外侧表面附近的簇中，位于前胸区和中胸区的边界，与受支配的肌纤维同侧。 MN1 和 2 的体细胞并排位于水平面上，其中 MN1 位于更靠前的位置。 MN3 和 4 的那些位于 MN1 和 2 的那些的腹侧，在水平面上，MN3 位于更靠前的位置。 MN5 的胞体位于其所支配的肌纤维的对侧，位于胸神经节最外层的背侧，靠近前胸区和中胸区边界的中线。尽管体体位于较远的对侧，但 MN5 的树突分布与 MN1-4 的树突分布在同一区域。根据这些结果，现在可以在银浸渍揭示的一般神经节结构中识别出这些神经元。讨论了 MN5 的个体发育对侧起源和所有五个 MN 的系统发育起源。
The dorsal longitudinal flight muscle (DLM) of Drosophila is composed of six muscle fibers (DLM1-6 from ventral to dorsal) and innervated by five motor neurons (MNs), DLM1-4 being innervated singly by MNI-4, and DLM5 and 6 being jointly innervated by MN5. This study identifies and describes the five motor neurons that innervate these six muscle fibers. Horseradish peroxidase (HRP) applied intracellularly to single identified DLM fibers resulted in the labeling of the single motor neuron innervating that muscle fiber by retrograde transsynaptic transport. This method allowed positive identification of the motor neuron innervating a particular muscle fiber, since only the innervating neuron was labeled. The axonal pathway, soma, and dendritic distribution of each labeled motor neuron was traced in the thoracic ganglion, and their relative positions were determined. The somata of MN1-4 lie in a cluster located near the lateral surface of the thoracic ganglion at the border of the pro- and mesothoracic regions, ipsilateral to the muscle fibers innervated. The somata of MN1 and 2 lie side by side in a horizontal plane with MN1 in a more anterior position. Those of MN3 and 4 lie ventrally to those of MN1 and 2 in a horizontal plane with MN3 in the more anterior position. The soma of MN5 is located contralaterally to the muscle fiber it innervates, lying in the dorsal of the outermost layer of the thoracic ganglion next to the midline at the border of the pro- and mesothoracic regions. In spite of the distant contralateral location of the soma, and dendritic distribution of MN5 occurs in the same area as those of MN1-4. From these results, these neurons can now be identified in the general ganglionic structure revealedby silver impregnation. The ontogenetic contralateral origin of MN5 and the phylogenetic origin of all five MNs are discussed.