Isolation and Characterization of a Distinct Influenza A Virus from Egyptian Bats
Isolation and Characterization of a Distinct Influenza A Virus from Egyptian Bats
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DOI：
10.1128/jvi.01059-18
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发表时间：
2019-01-01
影响因子：
5.4
通讯作者：
Ali, Mohamed A.
中科院分区：
文献类型：
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作者：
Kandeil, Ahmed;Goma, Mokhtar R.;Ali, Mohamed A.
最近，在危地马拉和秘鲁的蝙蝠身上发现了两种基因不同的流感病毒。我们在埃及的四种蝙蝠中进行了甲型流感病毒监测。在1,202份拭子标本中，105份经实时PCR检测呈阳性。用N-对甲苯磺酰-L-苯丙氨酸氯甲基酮处理的胰蛋白酶在鸡卵中成功地分离出一种病毒，并在MDCK细胞中繁殖。基因组分析显示，该病毒在遗传学上与所有其他甲型流感病毒不同。对血凝素基因的分析表明，该病毒与其他H9病毒有共同的祖先，而且该病毒与抗H9 N2病毒的血清的交叉反应性很低。蝙蝠对分离的病毒呈血清阳性。这种病毒在实验感染的小鼠的肺部复制。虽然它是遗传上不同的，这种病毒共享几个禽流感病毒的特点，建议最近的禽宿主origin.IMPORTANCE通过监测，我们分离和特点的流感A病毒从埃及果蝠。这种病毒对禽类受体有亲和力，但也能感染小鼠。我们的发现表明蝙蝠可能携带多种甲型流感病毒。这些病毒可能有可能跨越物种屏障感染其他物种，包括家禽、哺乳动物，也可能感染人类。
Recently, two genetically distinct influenza viruses were detected in bats in Guatemala and Peru. We conducted influenza A virus surveillance among four bat species in Egypt. Out of 1,202 swab specimens, 105 were positive by real-time PCR. A virus was successfully isolated in eggs and propagated in MDCK cells in the presence of N-tosyl-L-phenylalanine chloromethyl ketone-treated trypsin. Genomic analysis revealed that the virus was phylogenetically distinct from all other influenza A viruses. Analysis of the hemagglutinin gene suggested a common ancestry with other H9 viruses, and the virus showed a low level of cross-reactivity with serum raised against H9N2 viruses. Bats were seropositive for the isolated viruses. The virus replicated in the lungs of experimentally infected mice. While it is genetically distinct, this virus shares several avian influenza virus characteristics suggesting a more recent avian host origin.IMPORTANCE Through surveillance, we isolated and characterized an influenza A virus from Egyptian fruit bats. This virus had an affinity to avian-like receptors but was also able to infect mice. Our findings indicate that bats may harbor a diversity of influenza A viruses. Such viruses may have the potential to cross the species barrier to infect other species, including domestic birds, mammals, and, possibly, humans.