Collagen substrata for studies on cell behavior.
Collagen substrata for studies on cell behavior.
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DOI：
10.1083/jcb.54.3.626
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发表时间：
1972-09
期刊：
影响因子：
--
通讯作者：
Bard J
中科院分区：
文献类型：
--
作者：
Elsdale T;Bard J
本文描述了一种简单的技术，用于制备含有0.1%胶原蛋白（按重量计）的胶原蛋白基质，以具有640 A周期的天然束的形式，该基质在这些方面与软组织基质相似。这些基质是水合胶原蛋白晶格（hcl），其中水环境主要由毛细力保持在纤维胶原蛋白网内。从胶原沉淀和聚集过程、超微结构及其在各种条件下的稳定性等方面对hcl进行了表征。通过对人类二倍体细胞和两种恶性细胞系的形态、运动、粘附和生长的一些初步观察，说明了hcl在细胞行为研究中作为二维和三维基质的方法。
A simple technique is described for the preparation of collagen substrata containing 0 1% of collagen by weight, in the form of native bundles with a 640 A period, the substrata are similar in these respects to soft-tissue matrices These substrate are hydrated collagen lattices (HCLs) in which the watery milieu is held within a fibrous collagen net mainly by capillary forces. HCLs have been characterized in terms of the course of collagen precipitation and aggregation, ultrastructure, and their stability under various conditions. The ways in which HCLs can be employed as both two- and three-dimensional substrata in cell behavioral studies are illustrated with some preliminary observations on the form, motility, adhesion, and growth of human diploid cells and two lines of malignant cells.