Changes in galactosyltransferase activity in chick pectoral muscle during embryonic development.
Changes in galactosyltransferase activity in chick pectoral muscle during embryonic development.
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胚胎发育过程中小鸡胸肌半乳糖基转移酶活性的变化。
DOI：
10.1042/bj1960017
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Carlson,DM
中科院分区：
文献类型：
--
作者：
Ullrich,SJ;Kent,C;Carlson,DM
两种主要的脊椎动物半乳糖基转移酶已在卵内和体外以及培养的鸡成纤维细胞中对鸡肌肉的发育进行了研究。这两种酶是UDP-半乳糖-N-乙酰氨基葡萄糖半乳糖基转移酶（半乳糖基转移酶I）和UDP-半乳糖-N-乙酰基半乳糖胺半乳糖基转移酶（半乳糖基转移酶II）。这两项活动均在卵子肌肉发育过程中下降。半乳糖基转移酶 I 活性从第 7 天到第 16 天保持不变，此后下降了 5 倍，而半乳糖基转移酶 II 活性从第 9 天到第 13 天和第 16 天到第 20 天显着下降，总体下降了 8 倍。在原代肌肉培养物中，半乳糖基转移酶 I 活性在培养 7 天内略有下降，而半乳糖基转移酶 II 在同一时期增加了 2 倍。在细胞间识别和融合过程中，未观察到任一半乳糖基转移酶活性的显着变化。对用阿糖胞苷处理的肌肉培养物和成纤维细胞培养物的分析表明，原代肌肉培养物中的大多数半乳糖基转移酶 I 活性与成纤维细胞相关，而大多数半乳糖基转移酶 II 活性与肌肉相关。在原代肌肉培养物中添加 5-溴脱氧尿苷导致两种转移酶的活性增加 3 倍。
The two major vertebrate galactosyltransferases have been investigated in developing chick muscle in ovo and in vitro, and in cultured chick fibroblasts. The two enzymes were UDP-galactose-N-acetylglucosamine galactosyltransferase (galactosyltransferase I) and UDP-galactose-N-acetylgalactosamine galactosyltransferase (galactosyltransferase II). Both activities fell during muscle development in ovo. Galactosyltransferase I activity was constant from day 7 to day 16, after which it declined 5-fold, whereas galactosyltransferase II activity fell markedly from day 9 to 13 and 16 to 20, displaying an overall 8-fold decrease. In primary muscle cultures, galactosyltransferase I activity fell slightly during 7 days in culture, whereas galactosyltransferase II increased 2-fold during the same period. No significant change in activity of either galactosyltransferase was observed during intercellular recognition and fusion. Analysis of muscle cultures treated with cytosine arabinoside and of fibroblast cultures revealed that the majority of galactosyltransferase I activity in primary muscle cultures is associated with fibroblasts, whereas the majority of galactosyltransferase II activity is muscle-associated. The addition of 5-bromodeoxyuridine to primary muscle cultures resulted in a 3-fold rise in activities of both transferases.