Absolute and arbitrary orientation of single-molecule shapes
Absolute and arbitrary orientation of single-molecule shapes
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DOI：
10.1126/science.abd6179
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发表时间：
2021-02-19
期刊：
影响因子：
56.9
通讯作者：
Rothemund, Paul W. K.
中科院分区：
文献类型：
--
作者：
Gopinath, Ashwin;Thachuk, Chris;Rothemund, Paul W. K.
DNA折纸是一个模块化平台，用于分子和胶体成分的组合，以创建光学，电子和生物设备。这种纳米级器件与微制造连接器和电路的集成是具有挑战性的：大量的自由扩散器件必须以期望的对准方式固定在期望的位置。我们提出了一个DNA折纸分子，其能量景观光刻结合位点具有独特的最大值。该特性使器件在二氧化硅表面上的对准度在3.2度内。取向是绝对的（所有自由度都是指定的）和任意的（每个分子的取向都是独立指定的）。使用的取向，以优化器件的性能，通过对准荧光发射偶极子内的微制造的光学腔。大规模的整合证明了与3456 DNA折纸阵列与12个不同的方向，表明激发光的偏振。
DNA origami is a modular platform for the combination of molecular and colloidal components to create optical, electronic, and biological devices. Integration of such nanoscale devices with microfabricated connectors and circuits is challenging: Large numbers of freely diffusing devices must be fixed at desired locations with desired alignment. We present a DNA origami molecule whose energy landscape on lithographic binding sites has a unique maximum. This property enabled device alignment within 3.2 degrees on silica surfaces. Orientation was absolute (all degrees of freedom were specified) and arbitrary (the orientation of every molecule was independently specified). The use of orientation to optimize device performance was shown by aligning fluorescent emission dipoles within microfabricated optical cavities. Large-scale integration was demonstrated with an array of 3456 DNA origami with 12 distinct orientations that indicated the polarization of excitation light.