Presence of virulence factors in Enterococcus faecalis and Enterococcus faecium susceptible and resistant to vancomycin
Presence of virulence factors in Enterococcus faecalis and Enterococcus faecium susceptible and resistant to vancomycin
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DOI：
10.1590/s0074-02762013000500009
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发表时间：
2013-08-01
期刊：
影响因子：
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通讯作者：
d'Azevedo, Pedro Alves
中科院分区：
文献类型：
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作者：
Comerlato, Carolina Baldisserotto;Resende, Mariah Costa Carvalho de;d'Azevedo, Pedro Alves
尽管肠球菌作为机会性病原体的重要性日益增加，但对其毒力因素仍知之甚少。本研究确定了巴西阿雷格里港住院患者的临床和共生肠球菌分离株中毒力因子的频率。对 50 个肠球菌分离株进行了分析，并确定了 gelE、asa1 和 esp 基因的存在。还测试了明胶酶活性和生物膜形成。使用脉冲场凝胶电泳评估分离株之间的克隆关系。在所有分离株中，分别有 38%、60% 和 76% 发现了 asa1、gelE 和 esp 基因。前两个基因在粪肠球菌中比在屎肠球菌中更普遍，生物膜形成也是如此，与 gelE 和 asa1 基因相关，但与 esp 基因无关。 gelE 的存在和明胶酶的活性并不完全一致。在任何毒力因子和对万古霉素耐药的粪肠球菌或屎肠球菌的特定亚克隆之间没有观察到任何关系。总之，粪肠球菌和屎肠球菌分离株表现出显着不同的毒力决定因素模式。分离来源、克隆关系或万古霉素耐药性均不影响其分布。
Despite the increasing importance of Enterococcus as opportunistic pathogens, their virulence factors are still poorly understood. This study determines the frequency of virulence factors in clinical and commensal Enterococcus isolates from inpatients in Porto Alegre, Brazil. Fifty Enterococcus isolates were analysed and the presence of the gelE, asa1 and esp genes was determined. Gelatinase activity and biofilm formation were also tested. The clonal relationships among the isolates were evaluated using pulsed-field gel electrophoresis. The asa1, gelE and esp genes were identified in 38%, 60% and 76% of all isolates, respectively. The first two genes were more prevalent in Enterococcus faecalis than in Enterococcus faecium, as was biofilm formation, which was associated with gelE and asa1 genes, but not with the esp gene. The presence of gelE and the activity of gelatinase were not fully concordant. No relationship was observed among any virulence factors and specific subclones of E. faecalis or E. faecium resistant to vancomycin. In conclusion, E. faecalis and E. faecium isolates showed significantly different patterns of virulence determinants. Neither the source of isolation nor the clonal relationship or vancomycin resistance influenced their distribution.