RECEPTIVE FIELD ORGANIZATION OF BIPOLAR AND AMACRINE CELLS IN GOLDFISH RETINA
RECEPTIVE FIELD ORGANIZATION OF BIPOLAR AND AMACRINE CELLS IN GOLDFISH RETINA
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DOI：
10.1113/jphysiol.1973.sp010381
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发表时间：
1973-01-01
影响因子：
5.5
通讯作者：
KANEKO, A
中科院分区：
文献类型：
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作者：
KANEKO, A
1. 对分离的金鱼视网膜的双极细胞和无突细胞进行细胞内记录。参考以往细胞内Procion Yellow注射的研究成果，主要从细胞对点和环的反应模式来鉴定细胞。用白光和单色光分析记录细胞的感受野组织。所有的双相细胞在它们的感受野中都有一个中心-环绕组织。现场中心直径为100-200 μm，周围直径为1-1·5 mm。根据对单色光的响应特性，将双极细胞分为两类。对手的颜色细胞接收到来自红色和绿色视锥细胞的输入，以红色为中心，红色和绿色为外围，反之亦然。没有颜色编码的细胞接受来自视场中心和周围的红色视锥细胞的输入。在这些细胞中，中心和周围的平衡很好。无毛细胞也分为持续型和瞬时型两种类型。持续型无毛细胞响应稳定的电位变化，并以颜色编码。它们被红光超极化，被绿光去极化。瞬态型无突细胞对光闪的瞬态去极化响应。它们主要从红色视锥细胞接收信息，没有颜色编码。两种类型的无毛细胞在2.5 mm以上的区域内均表现出较大的空间总和；中心-周围拮抗未见。将感受野的大小与解剖学进行比较，特别是与树突扩散的大小进行比较，双极细胞的野中心与树突扩散的大小一致。双极细胞周围明显超过其树突场。由于双极细胞周围的响应特性与外部水平细胞的感受野最接近，因此，双极细胞周围的输入被认为是由外部水平细胞介导的。通过比较不同视网膜细胞的感受野特性，认为对手色双极细胞和颜色编码无突细胞都会聚到双对手神经节细胞。
1. Intracellular recordings were made from bipolar and amacrine cells in the isolated goldfish retina. Cells were identified mainly from their response patterns to a spot and an annulus in reference to the knowledge obtained from the previous work of intracellular Procion Yellow injection. Using white light and monochromatic lights receptive field organization of recorded cells were analysed.2. All bipolar cells had a centre‐surround organization in their receptive fields. The field centre was estimated to be 100–200 μm in diameter, and the surround 1–1·5 mm.3. Bipolar cells were classified into two types according to the response properties to monochromatic lights. Opponent colour cells received inputs from red and green cones, responding with red on‐centre, red and green off‐surround or vice versa. Cells without colour coding received input from red cones both in the field centre and the surround. In these cells the centre and the surround were well balanced.4. Amacrine cells were also classified into two types, a sustained type and a transient type. The sustained type amacrine cells responded with a steady potential change and were colour coded. They were hyperpolarized by red and depolarized by green light. The transient type amacrine cells responded with transient depolarization atonandoffof light flashes. They received input chiefly from red cones and were not colour coded. Both types of amacrine cells showed a large spatial summation in an area over 2·5 mm; centre‐surround antagonism was not seen.5. Comparing the size of the receptive field with anatomy, especially with the size of dendritic spread, the field centre of bipolar cells agreed in size with their dendritic spread. Bipolar cell surround clearly exceeded its dendritic field. Since the response properties of the bipolar cell surround was mimicked most closely by the receptive field of external horizontal cells, the input to the bipolar cell surround is thought to be mediated by external horizontal cells.6. By comparing receptive field properties of various retinal cells it is suggested that both the opponent colour bipolar cells and the colour coded amacrine cells converge on to the double opponent ganglion cells.