Free solution mobility of small single-stranded oligonucleotides with variable charge densities
Free solution mobility of small single-stranded oligonucleotides with variable charge densities
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DOI：
10.1002/elps.200305589
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发表时间：
2003-10-01
期刊：
影响因子：
2.9
通讯作者：
Stellwagen, NC
中科院分区：
文献类型：
--
作者：
Dong, Q;Stellwagen, E;Stellwagen, NC
已通过毛细管电泳测量了具有相同序列（包含多达 11 个中性氨基磷酸酯核苷间键）的 6 个单链 16 核苷酸 DNA 寡聚体的自由溶液迁移率。部分带电低聚物的迁移率随着电荷密度增加的对数线性增加，如曼宁电泳理论所预测的(G.S.Manning, J.Phys.Chem.1981,85,1506-1515)。为了进行比较，还测量了含有相似数量的带电磷酸残基的八种完全带电的单链寡聚物的迁移率。随着带电磷酸残基数量的增加，可变长度、完全带电的低聚物的迁移率比恒定大小、部分带电的氨基磷酸酯衍生物的迁移率增加得更快，因为修饰的低聚物的扩散系数更大。
The free solution mobilities of six single-stranded 16-nucleotide DNA oligomers with the same sequence, containing up to 11 neutral phosphoramidate internucleoside linkages, have been measured by capillary electrophoresis. The mobilities of the partially charged oligomers increase linearly with the logarithm of increasing charge density as, predicted by the Manning theory of electrophoresis (G. S. Manning, J. Phys. Chem. 1981, 85, 1506-1515). For comparison, the mobilities of eight fully charged single-stranded oligomers containing similar numbers of charged phosphate residues have also been measured. The mobilities of the variable length, fully charged oligomers increase more rapidly with the increasing number of charged phosphate residues than the mobilities of the constant size, partially charged phosphoramidate derivatives, because of the larger diffusion coefficients of the modified oligomers.