Distribution of Met-enkephalin-Arg6-Phe7 in various tissues of rats and Guinea pigs
Distribution of Met-enkephalin-Arg6-Phe7 in various tissues of rats and Guinea pigs
复制标题
Met-脑啡肽-Arg6-Phe7在大鼠和豚鼠各组织中的分布
DOI：
10.1016/0028-3908(82)90054-5
复制
发表时间：
1982
影响因子：
4.7
通讯作者：
E. Costa
中科院分区：
文献类型：
--
作者：
J. Tang;H. Y. Yang;E. Costa
用放射免疫法结合凝胶过滤和高效液相色谱法，对大鼠和豚鼠的胃、十二指肠、回肠、肌间神经丛、结肠、心、肺、胰腺、肝脏、肾上腺和颈上交感神经节中MEAP的存在和含量进行了测定。肠、肺各部位含量最高，颈上交感神经节、胰腺、肝脏几乎无免疫反应。在这些组织中，我们发现MEAP的分布与甲脑啡肽(ME)的分布不平行。我们的检测排除了大分子MEAP样多肽、心脏兴奋性四肽(Phe-Met-Arg-Phe NH2)和相应的酸Phe-Met-Arg-Phe的干扰。
A specific and sensitive radioimmunoassay coupled with gel filtration and HPLC was used to demonstrate the presence and to measure MEAP content in stomach, doudenum, ileum, myenteric plexus, colon, heart, lung, pancreas, liver, adrenal and superior cervical sympathetic ganglia of rat and guinea pig. The highest content was found in various parts of intestine, lung and superior cervical sympathetic ganglia, pancreas and liver were practically devoid of immunoreactivity. In these tissues we found that the distribution of MEAP is not parallel to that of metenkephalin (ME). Our assay excludes interferences by high molecular weight MEAPlike peptides, the cardioexcitatory tetrapeptide (Phe-Met-Arg-Phe NH2) and the correspondent acid Phe-Met-Arg-Phe.
影响因子：
5
作者：
Willette,RN;Sapru,HN
通讯作者：
Sapru,HN