The Prrx1eGFP Mouse Labels the Periosteum During Development and a Subpopulation of Osteogenic Periosteal Cells in the Adult.
The Prrx1eGFP Mouse Labels the Periosteum During Development and a Subpopulation of Osteogenic Periosteal Cells in the Adult.
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PRRX1EGFP小鼠在成年人中发育过程中标记骨膜的骨膜和成骨细胞的亚群。
DOI：
10.1002/jbm4.10707
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发表时间：
2023-03
期刊：
影响因子：
3.8
通讯作者：
Logan, Malcolm P. O.
中科院分区：
文献类型：
--
作者：
Brown, Sarah;Malik, Saif;Aljammal, Maria;O'Flynn, Aine;Hobbs, Carl;Shah, Mittal;Roberts, Scott J.;Logan, Malcolm P. O.
在发育过程中形成骨膜的细胞的身份是有争议的，目前的教条认为这些细胞来自Sox 9阳性祖细胞。在此，我们描述了一个新创建的Prrx1eGFP报告基因转基因小鼠在肢体形成和出生后。有趣的是，在胚胎中，Prrx1eGFP标记的细胞被限制在Sox9阳性软骨原基周围，而自身没有成为Sox9阳性。在成年人中，Prrx1eGFP转基因活标记骨膜内的细胞亚群，这些细胞在特定部位富集，而这一群体在老年小鼠中减少。绿色荧光蛋白（GFP）标记的亚群可使用荧光激活细胞分选（FACS）分离，约占所有分离骨膜细胞的8%。GFP标记的亚群比未标记的GFP阴性骨膜细胞成骨性显著更强。此外，骨膜细胞在体外的成骨和软骨形成能力可以通过向扩增培养基中添加成纤维细胞生长因子（FGF）来延长。我们提供的证据表明，胚胎中促进皮质骨形成的成骨细胞来源于软骨膜内的Prrx1eGFP阳性细胞，这些细胞可能与侵入的血管细胞相结合并分泌新的骨基质。总之，Prrx1eGFP小鼠是可视化和分离骨膜细胞并量化其在胚胎和成人中的特性的强大工具。© 2022作者JBMR Plus由Wiley Periodicals LLC代表美国骨与矿物质研究学会出版。
The identity of the cells that form the periosteum during development is controversial with current dogma suggesting these are derived from a Sox9‐positive progenitor. Herein, we characterize a newly created Prrx1eGFP reporter transgenic mouse line during limb formation and postnatally. Interestingly, in the embryo Prrx1eGFP‐labeled cells become restricted around the Sox9‐positive cartilage anlage without themselves becoming Sox9‐positive. In the adult, the Prrx1eGFP transgene live labels a subpopulation of cells within the periosteum that are enriched at specific sites, and this population is diminished in aged mice. The green fluorescent protein (GFP)‐labeled subpopulation can be isolated using fluorescence‐activated cell sorting (FACS) and represents approximately 8% of all isolated periosteal cells. The GFP‐labeled subpopulation is significantly more osteogenic than unlabeled, GFP‐negative periosteal cells. In addition, the osteogenic and chondrogenic capacity of periosteal cells in vitro can be extended with the addition of fibroblast growth factor (FGF) to the expansion media. We provide evidence to suggest that osteoblasts contributing to cortical bone formation in the embryo originate from Prrx1eGFP‐positive cells within the perichondrium, which possibly piggyback on invading vascular cells and secrete new bone matrix. In summary, the Prrx1eGFP mouse is a powerful tool to visualize and isolate periosteal cells and to quantify their properties in the embryo and adult. © 2022 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.
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影响因子：
2.1
作者：
Dwek, Jerry R.
通讯作者：
Dwek, Jerry R.
影响因子：
2.4
作者：
Benjamin, M;Toumi, H;Milz, S
通讯作者：
Milz, S
DOI：
10.1073/pnas.0504750102
发表时间：
2005-10-11
影响因子：
11.1
作者：
Akiyama, H;Kim, JE;de Crombrugghe, B
通讯作者：
de Crombrugghe, B
影响因子：
3.1
作者：
Asamura, Shinichi;Ikada, Yoshito;Isogai, Noritaka
通讯作者：
Isogai, Noritaka
影响因子：
4.5
作者：
Al Hosni, Rawiya;Shah, Mittal;Roberts, Scott J.
通讯作者：
Roberts, Scott J.