Homology of lysosomal enzymes and related proteins: Prediction of posttranslational modification sites including phosphorylation of mannose and potential epitopic and substrate binding sites in the α‐ and β‐subunits of hexosaminidases, α‐glucosidase, and rabbit and human isomaltase-猫眼课题宝

溶酶体酶和相关蛋白质的同源性：预测翻译后修饰位点，包括甘露糖的磷酸化以及己糖胺酶、α-葡萄糖苷酶、兔和人异麦芽糖酶的 α 和 β 亚基中的潜在表位和底物结合位点

DOI：

10.1002/prot.340040305

发表时间：

1988

期刊：

Proteins: Structure

影响因子：

通讯作者：

中科院分区：

文献类型：

作者：

Alison K. Barnes;C. Wynn

最近开发的计算机程序，包括二级结构和表位位点预测，已用于基于保守互换来比对溶酶体蛋白以获得最大同源性，并且已对比对序列进行搜索以寻找翻译后修饰、糖基化以及底物结合和催化的潜在位点。己糖胺酶的α和β亚基的翻译后修饰的同源性和预测与之前的观察结果非常一致，并且提出了对两个亚基的不同底物特异性的解释。我们表明，α-葡萄糖苷酶和异麦芽糖酶之间惊人的同源性反映在两种酶活性位点的明显对话中。在寻找糖原和麦芽糖的结合位点时已对非同源区域进行了详细检查，并已初步鉴定出两个这样的位点。建议采用高度冗余的溶酶体蛋白中甘露糖磷酸化共有序列 YXX（Y、W 或 F）。

Recently developed computer programs, including secondary structure and epitopic site predictions, have been used to align lysosomal proteins for maximum homology, based on conservative interchanges, and the aligned sequences have been searched for potential sites for posttranslational modification, glycosyaltion, and binding and catalysis of substrate. The homology and prediction of the posttranslational modification of the α‐ and β‐subunits of hexosaminidase is in good agreement with previous observations, and an explanation of the differing substrate specificities of the two subunits is advanced. We shows that the striking homology between α‐glucosidase and isomaltase is reflected in that apparent conversation of the active site in both enzymes. Nonhomologous regions have been examined in detail in a search for binding sited for glycogen and maltose, and two such sites have been tentatively identified. A highly redundant consensus sequence for the Phosphorylation of mannose in lysosomal proteins, YXX(Y, W, or F), is suggested.

Human beta-hexosaminidase alpha chain: coding sequence and homology with the beta chain.